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CTNNB1 抗体 (AA 29-49)

This anti-CTNNB1 antibody is a 兔 多克隆 antibody detecting CTNNB1 in WB, IP 和 ICC. Suitable for 人, 小鼠, 大鼠, 犬, 猴, 小鸡, Cow, 斑马鱼, 非洲爪蟾, Pig, 马, Hamster 和 Bat.
产品编号 ABIN211079
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CTNNB1 抗体 (AA 29-49) (ABIN211079)

抗原

See all CTNNB1 抗体
CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

适用

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人, 小鼠, 大鼠, 犬, 猴, 小鸡, Cow, 斑马鱼, 非洲爪蟾, Pig, 马, Hamster, Bat

宿主

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克隆类型

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多克隆

标记

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This CTNNB1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
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    AA 29-49

    特异性

    Recognizes b-Catenin, Mr 92kD. Species cross-reactivity: Human, bovine, rat, rabbit and mouse.

    预测反应

    Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%) Stickleback (90%).

    纯化方法

    Protein A purified

    免疫原

    Synthetic peptide corresponding to the consensus GSK3 phosphorylation site (aa29-49, CGG-SYLDSGIHSGATTTAPSLSGK) of human b-Catenin (KLH coupled). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%), Stickleback (90%).

    Type of Immunogen: Synthetic peptide - KLH conjugated

    亚型

    IgG
  • 应用备注

    Approved: ICC (1 - 5 μg/mL), IP, WB (0.1 - 2 μg/mL)

    Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 0.1-2 μg/mL. Detects b-catenin in an A431 total cell lysate. A431 total cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-b-Catenin (0.1 μg/mL). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg. Immunoprecipitates b-Catenin from 500 μg of A431 membrane fraction lysate. Immunocytochemistry: 1-5 μg/mL. Demonstrates positive immunostaining for b-catenin at cell-cell junctions of A431 cells fixed with 4 % paraformaldehyde and permeabilized with 0.25 % Triton X-100. Positive control: A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

    别名

    CTNNB1 / Beta Catenin

    背景

    Name/Gene ID: CTNNB1
    Family: Catenin

    Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

    基因ID

    1499

    UniProt

    P35222

    途径

    WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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