CTNNB1 抗体 (AA 29-49)
Quick Overview for CTNNB1 抗体 (AA 29-49) (ABIN211079)
抗原
See all CTNNB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 29-49
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特异性
- Recognizes b-Catenin, Mr 92kD. Species cross-reactivity: Human, bovine, rat, rabbit and mouse.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%) Stickleback (90%).
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纯化方法
- Protein A purified
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免疫原
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Synthetic peptide corresponding to the consensus GSK3 phosphorylation site (aa29-49, CGG-SYLDSGIHSGATTTAPSLSGK) of human b-Catenin (KLH coupled). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Pufferfish, Zebrafish (100%), Stickleback (90%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
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anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody
KO Validated CTNNB1 适用: 人 WB, IHC, IF, FACS, IHC (p), IP, ICC, IHC (fro), ChIP, CUT&RUN, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibodyCTNNB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (AA 2-781) antibodyCTNNB1 适用: 人 WB, IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (AA 682-781) antibodyCTNNB1 适用: 人 WB, ELISA, IF, PLA, RNAi 宿主: 小鼠 Monoclonal 1C9 unconjugated
anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) antibodyCTNNB1 适用: 人 WB, IHC, IF, FACS, StM 宿主: 兔 Monoclonal CTNNB1-2030R unconjugated Recombinant Antibody
anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) antibodyCTNNB1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 9C1 unconjugated
anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) antibodyCTNNB1 适用: 人, 猴 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 6A3 unconjugated
anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) antibodyCTNNB1 适用: 人, 大鼠, 犬, 猴 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 9H2 unconjugated
anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) antibodyCTNNB1 适用: 人, 大鼠, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 12H7 unconjugated
anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) antibodyCTNNB1 适用: 人, 犬 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1E10 unconjugated
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应用备注
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Approved: ICC (1 - 5 μg/mL), IP, WB (0.1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 0.1-2 μg/mL. Detects b-catenin in an A431 total cell lysate. A431 total cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-b-Catenin (0.1 μg/mL). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg. Immunoprecipitates b-Catenin from 500 μg of A431 membrane fraction lysate. Immunocytochemistry: 1-5 μg/mL. Demonstrates positive immunostaining for b-catenin at cell-cell junctions of A431 cells fixed with 4 % paraformaldehyde and permeabilized with 0.25 % Triton X-100. Positive control: A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
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别名
- CTNNB1 / Beta Catenin
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背景
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Name/Gene ID: CTNNB1
Family: Catenin
Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin -
基因ID
- 1499
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UniProt
- P35222
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途径
- WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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