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ZBTB33 抗体 (AA 103-584)

This anti-ZBTB33 antibody is a 小鼠 单克隆 antibody detecting ZBTB33 in WB, IP 和 IHC. Suitable for 人, 小鼠, 大鼠, 犬, 小鸡 和 Hamster.
产品编号 ABIN210906
发货至: 中国

Quick Overview for ZBTB33 抗体 (AA 103-584) (ABIN210906)

抗原

See all ZBTB33 抗体
ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))

适用

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人, 小鼠, 大鼠, 犬, 小鸡, Hamster

宿主

  • 22
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小鼠

克隆类型

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单克隆

标记

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This ZBTB33 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
  • 抗原表位

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    AA 103-584

    特异性

    Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.

    纯化方法

    Protein G purified

    免疫原

    Recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).

    Type of Immunogen: Recombinant protein

    亚型

    IgG1
  • 应用备注

    Approved: IHC (1 μg/mL), IP, WB (0.5 - 2 μg/mL)

    Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 μg/mL gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100 % methanol. Western Blot: 0.5-2 μg/mL detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 μg immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))

    别名

    ZBTB33 / Kaiso

    背景

    Name/Gene ID: ZBTB33

    Synonyms: ZBTB33, Kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, Kaiso transcription factor, ZNF348

    基因ID

    10009

    UniProt

    Q86T24
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