ZBTB33 抗体 (AA 103-584)
Quick Overview for ZBTB33 抗体 (AA 103-584) (ABIN210906)
抗原
See all ZBTB33 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 103-584
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特异性
- Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.
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纯化方法
- Protein G purified
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免疫原
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Recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).
Type of Immunogen: Recombinant protein -
亚型
- IgG1
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应用备注
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Approved: IHC (1 μg/mL), IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 μg/mL gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100 % methanol. Western Blot: 0.5-2 μg/mL detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 μg immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))
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别名
- ZBTB33 / Kaiso
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背景
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Name/Gene ID: ZBTB33
Synonyms: ZBTB33, Kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, Kaiso transcription factor, ZNF348 -
基因ID
- 10009
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UniProt
- Q86T24
抗原
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