我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

OCT4 抗体 (AA 282-297)

Name: OCT4 抗体 (AA 282-297)
SKU: ABIN209117
Availability: OutOfStock

This anti-OCT4 antibody is a 兔多克隆 antibody detecting OCT4 in WB, IHC 和 ICC. Suitable for 人和 Rodents.

产品编号 ABIN209117

发货至: 中国

Quick Overview for OCT4 抗体 (AA 282-297) (ABIN209117)

抗原

See all OCT4 (POU5F1) 抗体

OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

适用

人, Rodents

宿主

兔

克隆类型

多克隆

标记

This OCT4 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Discover our top product POU5F1 Primary Antibody

抗原表位
16

10

10

8

6

5

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 282-297

特异性

Recognizes Oct4 (POU5F1). MW: 38,.6kD. Species cross-reactivity: Human and rodent.

纯化方法

Immunoaffinity purified

免疫原

Synthetic peptide (aa 282-297) of Oct4.

Type of Immunogen: Synthetic peptide

亚型

IgG
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
KO Validated POU5F1 适用: 人 WB, IF, ICC, IP, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 201-300) antibody
POU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibody
POU5F1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2H41D8 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E7 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 136-360) antibody
POU5F1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibody
POU5F1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (Internal Region) antibody
POU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Approved: ICC (1:200 - 1:1000), IHC (1:200 - 1:1000), WB (1:200 - 1:1000)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 using ECL. Immunohistochemistry: 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.Immunohistochemistry protocol:1. Dissect tissues and freeze on dry ice.2. Cut on a cryostat 10um slices.3. Dry slides at RT for 30-90 min4. Fix with cold Acetone: Methanol (50:50) 2 min. Either process further or store at -20 °C. Alternative is fixation with 4 % PFA and treatment with 0.05 % trypsin solution (incubation 10 min. at 37 °C, followed by 3 washes with PBS)5. Let air dry.6. PBS 5 min7. Incubate in 50  mM ammonium chloride 30 min.8. PBS 5 min9. Blocking serum 30-45 min10. Primary antibody (LS-C9379) 90 min11. Wash 3X for 5 min12. Secondary fluorescent conjugated antibody 30 min13. Wash 3X for 5 min.14. Mount.

说明

Target Species of Antibody: Human

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

PBS,  pH 7.4. No preservative added.

储存液

Without preservative

注意事项

avoid freeze thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
抗原

OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

别名

POU5F1 / OCT4

背景

Name/Gene ID: POU5F1

Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3

基因ID

5460

UniProt

Q01860

途径

Stem Cell Maintenance