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OCT4 抗体 (AA 282-297)

Name: OCT4 抗体 (AA 282-297)
SKU: ABIN209117
Availability: OutOfStock

The 兔多克隆 anti-OCT4 antibody is suitable to detect OCT4 in samples from 人和 Rodents. It has been validated for WB, IHC 和 ICC.

产品编号 ABIN209117

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for OCT4 抗体 (AA 282-297) (ABIN209117)

抗原

See all OCT4 (POU5F1) 抗体

OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

适用

人, Rodents

宿主

兔

克隆类型

多克隆

标记

This OCT4 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Discover our top product POU5F1 Primary Antibody

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AA 282-297

特异性

Recognizes Oct4 (POU5F1). MW: 38,.6kD. Species cross-reactivity: Human and rodent.

纯化方法

Immunoaffinity purified

免疫原

Synthetic peptide (aa 282-297) of Oct4.

Type of Immunogen: Synthetic peptide

亚型

IgG
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
KO Validated POU5F1 适用: 人 WB, ICC, IP, IF, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 201-300) antibody
POU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibody
POU5F1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2H41D8 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E7 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 136-360) antibody
POU5F1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibody
POU5F1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB1425 unconjugated Recombinant Antibody

应用备注

Approved: ICC (1:200 - 1:1000), IHC (1:200 - 1:1000), WB (1:200 - 1:1000)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 using ECL. Immunohistochemistry: 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.Immunohistochemistry protocol:1. Dissect tissues and freeze on dry ice.2. Cut on a cryostat 10um slices.3. Dry slides at RT for 30-90 min4. Fix with cold Acetone: Methanol (50:50) 2 min. Either process further or store at -20 °C. Alternative is fixation with 4 % PFA and treatment with 0.05 % trypsin solution (incubation 10 min. at 37 °C, followed by 3 washes with PBS)5. Let air dry.6. PBS 5 min7. Incubate in 50  mM ammonium chloride 30 min.8. PBS 5 min9. Blocking serum 30-45 min10. Primary antibody (LS-C9379) 90 min11. Wash 3X for 5 min12. Secondary fluorescent conjugated antibody 30 min13. Wash 3X for 5 min.14. Mount.

说明

Target Species of Antibody: Human

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

PBS,  pH 7.4. No preservative added.

储存液

Without preservative

注意事项

avoid freeze thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
抗原

OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

别名

POU5F1 / OCT4

背景

Name/Gene ID: POU5F1

Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3

基因ID

5460

UniProt

Q01860

途径

Stem Cell Maintenance