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IRS1 抗体 (AA 1222-1235)

This 兔 多克隆 antibody specifically detects IRS1 in WB 和 IP. It exhibits reactivity toward 大鼠.
产品编号 ABIN208882
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IRS1 抗体 (AA 1222-1235) (ABIN208882)

抗原

See all IRS1 抗体
IRS1 (Insulin Receptor Substrate 1 (IRS1))

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This IRS1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP)
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    AA 1222-1235

    特异性

    Recognizes IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.

    预测反应

    Percent identity by BLAST analysis: Rat (100%) Human, Gorilla, Mouse, Hamster, Rabbit (93%) Marmoset, Chicken (86%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic peptide corresponding to 14 amino acid ([C]YASINFQKQPEDRQ) with in the carboxy terminal of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%), Human, Gorilla, Mouse, Hamster, Rabbit (93%), Marmoset, Chicken (86%).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: IP, WB (0.5 - 2 μg/mL)

    Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 μg/mL. Immunoprecipitation: 4 μg immunoprecipitates IRS-1 from 500 μg of 3T3/A31 RIPA lysate. Positive control: Fibroblast Cell, 3T3/A31, L6 myoblasts.

    说明

    Target Species of Antibody: Rat

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.2 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 5 mg/mL BSA, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    IRS1 (Insulin Receptor Substrate 1 (IRS1))

    别名

    IRS1 / IRS-1

    背景

    Name/Gene ID: IRS1

    Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1

    基因ID

    3667

    UniProt

    P35568

    途径

    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
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