IRS1 抗体 (AA 1222-1235)
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北京 101111
Quick Overview for IRS1 抗体 (AA 1222-1235) (ABIN208882)
抗原
See all IRS1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1222-1235
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特异性
- Recognizes IRS-1 at 160-185kD. Species cross-reactivity: Human, mouse and rat.
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预测反应
- Percent identity by BLAST analysis: Rat (100%) Human, Gorilla, Mouse, Hamster, Rabbit (93%) Marmoset, Chicken (86%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide corresponding to 14 amino acid ([C]YASINFQKQPEDRQ) with in the carboxy terminal of rat liver IRS-1. Percent identity by BLAST analysis: Rat (100%), Human, Gorilla, Mouse, Hamster, Rabbit (93%), Marmoset, Chicken (86%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots detected IRS-1 in RIPA lysates of rat L6 myoblasts. In cells that contain high levels of IRS-1, this lot may work optimally at 0.1-0.5 μg/mL. Immunoprecipitation: 4 μg immunoprecipitates IRS-1 from 500 μg of 3T3/A31 RIPA lysate. Positive control: Fibroblast Cell, 3T3/A31, L6 myoblasts. -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 5 mg/mL BSA, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- IRS1 (Insulin Receptor Substrate 1 (IRS1))
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别名
- IRS1 / IRS-1
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背景
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Name/Gene ID: IRS1
Synonyms: IRS1, IRS-1, HIRS-1, Insulin receptor substrate 1 -
基因ID
- 3667
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UniProt
- P35568
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
抗原
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