Vimentin 抗体
Quick Overview for Vimentin 抗体 (ABIN204914)
抗原
See all Vimentin (VIM) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Mesenchymal cells and mesenchymal derived tumors e. g. lymphoma, sarcoma and melanoma. Reacts with the 57kD protein corresponding to vimentin. Species cross-reactivity: Human, rat, chicken, potoroo.
-
纯化方法
- Protein G purified
-
免疫原
-
Vimentin.
Type of Immunogen: Purified protein -
亚型
- IgG1
-
-
-
-
应用备注
-
Approved: Flo, ICC, IHC, IHC-Fr, IHC-P, WB (1:100 - 1:1000)
Usage: Suitable for use in Immunohistochemistry, Flow Cytometry, Western Blot and Immunocytochemistry. Immunohistochemistry (Frozen, Paraffin): 1:25-1:200 using ABC method. Paraffin sections require HIER with 10 mM citrate buffer, pH 6.0, for 10 min. Cool sections at room temperature for 20 min. Flow Cytometry: 1-25-1:200. Western Blot: 1:100-1:1000. Optimum dilutions to be determined by researcher. Hybridoma: PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from porcine lens. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, pH 7.2, 0.1 % sodium azide. No stabilizing proteins added.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
-
-
- Vimentin (VIM)
-
别名
- Vimentin
-
背景
-
Name/Gene ID: VIM
Synonyms: VIM, Vimentin -
基因ID
- 7431
-
UniProt
- P08670
-
途径
- Caspase Cascade in Apoptosis
抗原
-