SOD2 抗体
Quick Overview for SOD2 抗体 (ABIN204760)
抗原
See all SOD2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human MnSOD. Species cross-reactivity: rat.
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纯化方法
- Protein G purified
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免疫原
- Manganese superoxide dismutase (MnSOD).
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亚型
- IgG1
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应用备注
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Approved: ICC, IHC, IHC-Fr (0.5 - 10 μg/mL), WB
Usage: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Immunohistochemistry (Acetone fixed cryostat sections): 0.5-10 μg/mL using an APAAP or PAP procedure. Antibody also reacts with 4 % PFA fixed frozen tissue. Typical block contains 1 % non-fat dry milk or 3 % NGS and 0.2-1.5 % triton X-100 (higher triton is only used on thick sections, blocked for 1-3 hours at RT. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk), typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4?C. Wash 3x10' in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites. Immunocytochemistry (cell smears): 0.5-10 μg/mL. Western Blot: Under reducing conditions a band at ~25kD will be identified. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))
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别名
- MNSOD / SOD2
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背景
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Name/Gene ID: SOD2
Synonyms: SOD2, IPOB, MVCD6, Indophenoloxidase B, Mn superoxide dismutase, MNSOD, Mangano-superoxide dismutase -
基因ID
- 6648
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UniProt
- P04179
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途径
- Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling
抗原
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