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IL17D 抗体 (AA 18-202)

This anti-IL17D antibody is a 小鼠 单克隆 antibody detecting IL17D in ELISA, WB, IHC, FACS 和 IHC (p). Suitable for 人.
产品编号 ABIN204351
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IL17D 抗体 (AA 18-202) (ABIN204351)

抗原

See all IL17D 抗体
IL17D (Interleukin 17D (IL17D))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This IL17D antibody is un-conjugated

应用范围

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ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

246018
  • 抗原表位

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    AA 18-202

    特异性

    Recognizes human IL-27. Does not cross-react with rhIL-17, rhIL17B, rhIL-17C, rhIL-17E, or rhIL-17F. Species sequence homology: Mouse: 86 % .

    纯化方法

    Protein G purified

    免疫原

    E. coli-derived recombinant human Interleukin 27 (aa18-202).

    亚型

    IgG2b
  • 应用备注

    Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-P, WB (1 - 2 μg/mL)

    Usage: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and ELISA. Western Blot: 1-2 μg/mL. Detection limit is approximately 25 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Flow Cytometry: 25 μg/mL. For intracellular staining, cells must be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Add 10 μL of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. Incubate for 30 minutes and then wash cells with 0.1 % saponin prior to addition of a secondary developing reagent. Cells should be washed a final time in 0.1 % saponin prior to flow cytometric analysis. Direct ELISA: 0.5-1 μg/mL. Detection limit is ~5 ng/well. Immunohistochemistry: 8-25 μg/mL with appropriate secondary reagents to detect IL-17D in paraffin-embedded normal heart tissue sections.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute with sterile 40-50 % glycerol, PBS.

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS, 5 % trehalose.

    储存条件

    -20 °C

    储存方法

    Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.
  • 抗原

    IL17D (Interleukin 17D (IL17D))

    别名

    IL17D

    背景

    Name/Gene ID: IL17D
    Family: Interleukin

    Synonyms: IL17D, IL-27, Interleukin 27, Interleukin-17D, Interleukin-27, IL-17D, IL-22, Interleukin 17D

    基因ID

    53342

    UniProt

    Q8TAD2

    途径

    Cellular Response to Molecule of Bacterial Origin
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