IL17D 抗体 (AA 18-202)
Quick Overview for IL17D 抗体 (AA 18-202) (ABIN204351)
抗原
See all IL17D 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 18-202
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特异性
- Recognizes human IL-27. Does not cross-react with rhIL-17, rhIL17B, rhIL-17C, rhIL-17E, or rhIL-17F. Species sequence homology: Mouse: 86 % .
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纯化方法
- Protein G purified
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免疫原
- E. coli-derived recombinant human Interleukin 27 (aa18-202).
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亚型
- IgG2b
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-P, WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and ELISA. Western Blot: 1-2 μg/mL. Detection limit is approximately 25 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Flow Cytometry: 25 μg/mL. For intracellular staining, cells must be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Add 10 μL of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. Incubate for 30 minutes and then wash cells with 0.1 % saponin prior to addition of a secondary developing reagent. Cells should be washed a final time in 0.1 % saponin prior to flow cytometric analysis. Direct ELISA: 0.5-1 μg/mL. Detection limit is ~5 ng/well. Immunohistochemistry: 8-25 μg/mL with appropriate secondary reagents to detect IL-17D in paraffin-embedded normal heart tissue sections. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with sterile 40-50 % glycerol, PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose.
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储存条件
- -20 °C
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储存方法
- Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.
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- IL17D (Interleukin 17D (IL17D))
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别名
- IL17D
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背景
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Name/Gene ID: IL17D
Family: Interleukin
Synonyms: IL17D, IL-27, Interleukin 27, Interleukin-17D, Interleukin-27, IL-17D, IL-22, Interleukin 17D -
基因ID
- 53342
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UniProt
- Q8TAD2
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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