NOS1 抗体 (AA 724-739)
Quick Overview for NOS1 抗体 (AA 724-739) (ABIN201344)
抗原
See all NOS1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 724-739
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特异性
- Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.
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纯化方法
- Affinity purified
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免疫原
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Ved in human, rat and mouse. IMMUNOGEN: Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, IF, IHC, WB (1 - 10 μg/mL)
Usage: Immunohistochemistry: Use at 5-10 μg/mL on rat neuronal sections fixed with 3.5 % paraformaldehyde. Incubation overnight at 4?C with a 1:100-1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons. Western blot: 1-10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155kD protein. ELISA: Use at 1.0 μg/mL or lower on plates coated with 50-100 ng of peptide antigen. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % BSA, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))
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别名
- NOS1 / nNOS
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背景
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Name/Gene ID: NOS1
Synonyms: NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i -
基因ID
- 4842
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UniProt
- P29475
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途径
- Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction
抗原
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