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NOS1 抗体 (AA 724-739)

The 兔 多克隆 anti-NOS1 antibody is suitable to detect NOS1 in samples from 人. It has been validated for WB, IHC, ELISA 和 IF.
产品编号 ABIN201344
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for NOS1 抗体 (AA 724-739) (ABIN201344)

抗原

See all NOS1 抗体
NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

适用

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宿主

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克隆类型

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多克隆

标记

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This NOS1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
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    AA 724-739

    特异性

    Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.

    纯化方法

    Affinity purified

    免疫原

    Ved in human, rat and mouse. IMMUNOGEN: Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.

    Type of Immunogen: Synthetic peptide

    亚型

    IgG
  • 应用备注

    Approved: ELISA, IF, IHC, WB (1 - 10 μg/mL)

    Usage: Immunohistochemistry: Use at 5-10 μg/mL on rat neuronal sections fixed with 3.5 % paraformaldehyde. Incubation overnight at 4?C with a 1:100-1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons. Western blot: 1-10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155kD protein. ELISA: Use at 1.0 μg/mL or lower on plates coated with 50-100 ng of peptide antigen.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.1 % BSA, 0.1 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

    别名

    NOS1 / nNOS

    背景

    Name/Gene ID: NOS1

    Synonyms: NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i

    基因ID

    4842

    UniProt

    P29475

    途径

    Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction
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