BUB1B 抗体 (AA 1-350)
Quick Overview for BUB1B 抗体 (AA 1-350) (ABIN199935)
抗原
See all BUB1B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-350
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特异性
- Amino acid residues 1-350 of human BUBR1 Kinase protein.
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纯化方法
- Protein A purified
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免疫原
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Recombinant human BUB1B / BubR1.
Type of Immunogen: Recombinant protein
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应用备注
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Approved: IF, IP, WB
Usage: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~120 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2 % Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1 % BSA.
Not recommended for: IHC-P -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))
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别名
- BUB1B / BubR1
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背景
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Name/Gene ID: BUB1B
Subfamily: BUB1
Family: Protein Kinase
Synonyms: BUB1B, Bub1A, BUBR1, HBUBR1, Protein SSK1, SSK1, BUB1beta, MAD3L -
基因ID
- 701
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UniProt
- O60566
抗原
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