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TYR 抗体 (AA 1-433)

This 小鼠 单克隆 antibody specifically detects TYR in WB, IHC, IF, IHC (p) 和 ICC. It exhibits reactivity toward 人, 犬 和 Cat.
产品编号 ABIN199780
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for TYR 抗体 (AA 1-433) (ABIN199780)

抗原

See all TYR 抗体
TYR (Tyrosinase (TYR))

适用

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人, 犬, Cat

宿主

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小鼠

克隆类型

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单克隆

标记

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This TYR antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
  • 抗原表位

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    AA 1-433

    特异性

    Recognizes a cluster of proteins between 70-80kD, identified as tyrosinase. Occasionally a minor band at 55kD is also detected. Does not cross-react with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species cross-reactivity: Human, canine and feline. Weak cross-reactivity with mouse.

    纯化方法

    Protein A purified

    免疫原

    Recombinant tyrosinase protein. Epitope: 1-433. Cellular Localization: Cytoplasmic.

    Type of Immunogen: Recombinant protein

    亚型

    IgG2a
  • 应用备注

    Approved: ICC, IF, IHC, IHC-P, WB (1 - 2 μg/mL)

    Usage: Suitable for use in Immunocytology, Immunofluorescence, Western Blot and Immunohistology. Western Blot: 1-2 μg/mL for 2 hours at RT. Immunohistology: 2-4 μg/mL for 30 minutes at RT (formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA,  pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Melanoma cell lines (SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37), melanocytes in skin and Melanomas (1). The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4. No preservatives or stabilizing proteins added.

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    TYR (Tyrosinase (TYR))

    别名

    Tyrosinase

    背景

    Name/Gene ID: TYR

    Synonyms: TYR, CMM8, LB24-AB, SHEP3, SK29-AB, Monophenol monooxygenase, Tumor rejection antigen AB, OCA1A, Tyrosinase, OCAIA

    基因ID

    7299

    UniProt

    P14679
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