Perforin 1 抗体 (PE)
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北京 101111
Quick Overview for Perforin 1 抗体 (PE) (ABIN1981897)
抗原
See all Perforin 1 (PRF1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Perforin PE
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无交叉反应
- 小鼠
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交叉反应 （详细）
- Human, Bovine
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产品特性
- The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
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纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- purified granules from human YT lymphoma cell line
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亚型
- IgG2b, kappa
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anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 280-555) antibody
PRF1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibodyPRF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 461-555) antibodyPRF1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3B4 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibodyPRF1 适用: 人, Cow IP, FACS, ICC, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal dG9 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 40-355) antibodyPRF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 413-552) antibodyPRF1 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal PRF1-2470 unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (FITC)PRF1 适用: 人, Cow ICFC 宿主: 小鼠 Monoclonal dG9 FITC
anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (APC)PRF1 适用: 人, Cow ICFC 宿主: 小鼠 Monoclonal dG9 APC
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 32-316) antibodyPRF1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 21-554) antibodyPRF1 适用: 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze,Protect from prolonged exposure to light
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." in: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, (2012) (PubMed).
: "Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT." in: Journal of molecular cell biology, Vol. 2, Issue 3, pp. 139-51, (2010) (PubMed).
: "Flow cytometric detection of perforin upregulation in human CD8 T cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 73, Issue 11, pp. 1050-7, (2008) (PubMed).
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: "Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." in: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, (2012) (PubMed).
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- Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))
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别名
- Perforin
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背景
- Perforin 1,Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.,PRF1, P1, PFP, HPLH2
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基因ID
- 5551
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UniProt
- P14222
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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