HSF1 抗体 (Ser307)
Quick Overview for HSF1 抗体 (Ser307) (ABIN197434)
抗原
See all HSF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Ser307
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特异性
- HSF1 antibody detects endogenous levels of total HSF1 protein.
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纯化方法
- Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- The antiserum was produced against synthesized non-phosphopeptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-SP-P-R).
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anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (HRP)
HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 HRP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (FITC)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 FITC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Biotin)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Biotin
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PE)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PE
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (APC)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 APC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PerCP)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 PerCP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 488)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 488
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 594)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 594
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 390)HSF1 适用: 小鼠 WB, ELISA, IHC, IP, IF, GS, ICC 宿主: 大鼠 Monoclonal 10H8 Atto 390
anti-Heat Shock Factor Protein 1 (HSF1) (AA 350-529) antibodyHSF1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry on paraffin sections: 1: 50-1: 100.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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别名
- Heat Shock Factor 1 / HSF1
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背景
- All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38 % sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1
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基因ID
- 3297
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NCBI登录号
- NP_005517
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UniProt
- Q00613
抗原
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