PRKAA1 抗体 (Thr174)
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Quick Overview for PRKAA1 抗体 (Thr174) (ABIN197410)
抗原
See all PRKAA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Thr174
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特异性
- This antibody AP02704PU detects endogenous levels of total AMPK alpha-1/AMPK alpha-2 protein.
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纯化方法
- Immunoaffinity Chromatography using epitope-specific immunogen.
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免疫原
- The antiserum was produced against synthesized non-phosphopeptide derived from human AMPK1 alpha-1 around the phosphorylation site of threonine 174 (L-R-Tp-S-C).
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应用备注
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))
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别名
- PRKAA1
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背景
- Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-1/2, AMPK alpha-1, AMPK alpha-2, AMPK1, AMPK2, PRKAA1, PRKAA2
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基因ID
- 5562
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NCBI登录号
- NP_006242
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UniProt
- Q13131
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途径
- AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect
抗原
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