CAMK2A 抗体 (pThr286)
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Quick Overview for CAMK2A 抗体 (pThr286) (ABIN197054)
抗原
See all CAMK2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr286
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特异性
- This antibody detects endogenous levels of CaMKII only when phosphorylated at Threonine 286.
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纯化方法
- Affinity-Chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
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免疫原
- The antiserum was produced against synthesized phosphopeptide derived from Human CaMKII around the phosphorylation site of Threonine 286 (Q-E-Tp-V-D).
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应用备注
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Western Blot: 1/500approx. 1/1000. Incubate membrane with diluted antibody in 5 % nonfat milk, 1X TBS, 0.1 % Tween-20 at 4 °Cwith gentle shaking, overnight. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
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别名
- CAMK2A
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背景
- CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968
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基因ID
- 815
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NCBI登录号
- NP_741960
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UniProt
- Q9UQM7
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途径
- WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
抗原
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