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CAMK2A 抗体 (pThr286)

The 兔 多克隆 anti-CAMK2A antibody is suitable to detect CAMK2A in samples from 人, 小鼠 和 大鼠. It has been validated for WB 和 IF.
产品编号 ABIN197054
发货至: 中国
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Quick Overview for CAMK2A 抗体 (pThr286) (ABIN197054)

抗原

See all CAMK2A 抗体
CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

  • 62
  • 49
多克隆

标记

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This CAMK2A antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF)
  • 抗原表位

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    pThr286

    特异性

    This antibody detects endogenous levels of CaMKII only when phosphorylated at Threonine 286.

    纯化方法

    Affinity-Chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

    免疫原

    The antiserum was produced against synthesized phosphopeptide derived from Human CaMKII around the phosphorylation site of Threonine 286 (Q-E-Tp-V-D).
  • 应用备注

    Western Blot: 1/500approx. 1/1000. Incubate membrane with diluted antibody in 5 % nonfat milk, 1X TBS, 0.1 % Tween-20 at 4 °Cwith gentle shaking, overnight. Immunofluorescence: 1/100approx. 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the antibody (in aliquots) at -20 °C.
  • 抗原

    CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

    别名

    CAMK2A

    背景

    CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968

    基因ID

    815

    NCBI登录号

    NP_741960

    UniProt

    Q9UQM7

    途径

    WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
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