The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. SMC1 (phospho-Ser957) antibody detects endogenous levels of SMC1 only when phosphorylated at serine 957.
纯化方法
Immunoaffinity chromatography using epitope-specific phosphopeptide.
免疫原
The antiserum was produced against synthesized phosphopeptide derived from human SMC1 around the phosphorylation site of serine 957 (G-S-Sp-Q-G).
Suitable for use in Suitable for use in Western blot (1: 500-1: 1000) andImmunohistochemistry on paraffin-embedded sections (1: 50-1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody (in aliquots) at -20 °C.
抗原
SMC1A
(Structural Maintenance of Chromosomes 1A (SMC1A))