RPS6KA1 抗体 (pThr348)
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Quick Overview for RPS6KA1 抗体 (pThr348) (ABIN196796)
抗原
See all RPS6KA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr348
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特异性
- This antibody detects endogenous levels of p90RSK only when phosphorylated at Threonine 348.
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纯化方法
- Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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免疫原
- The antiserum was produced against synthesized phosphopeptide derived from mouse p90RSK around the phosphorylation site of threonine 348 (S-R-TP-P-R).
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应用备注
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Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
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别名
- RPS6KA1
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背景
- Synonyms: 90 kDa ribosomal protein S6 kinase 1, MAPKAPK1A, RSK-1, RSK1, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase alpha-1, p90RSK1
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基因ID
- 6195
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NCBI登录号
- NP_001006666
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UniProt
- Q15418
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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