The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Myc (phospho-Ser373) antibody detects endogenous levels of Myc only when phosphorylated at serine 373.
纯化方法
Immunoaffinity chromatography.
免疫原
The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of serine 373.
Suitable for use in Immunohistochemistry (1: 50approx. 1: 100). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.