ELK1 抗体 (pSer383)
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- 抗原 See all ELK1 抗体
- ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))
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抗原表位
- pSer383
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ELK1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed bychromatography using non-phosphopeptide corresponding to the phosphorylation site. Elk-1 (phospho-Ser383) antibody detects endogenous levels of Elk-1 only when phosphorylated at serine 383.
- 纯化方法
- Immunoaffinity chromatography.
- 免疫原
- The antiserum was produced against synthesized phosphopeptide derived from human Elk-1 around the phosphorylation site of Serine 383 (T-L-S(p)-P-I).
- Top Product
- Discover our top product ELK1 Primary Antibody
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anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer383) antibody
ELK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer389) antibodyELK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (C-Term) antibodyELK1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) antibodyELK1 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 3H6D12 unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer383) antibodyELK1 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (AA 247-403) antibodyELK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pThr417) antibodyELK1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer389) antibodyELK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer383) antibodyELK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ELK1, Member of ETS Oncogene Family (ELK1) (AA 67-166) antibodyELK1 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 2G6 unconjugated
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- 应用备注
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Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody (in aliquots) at -20 °C.
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- 抗原
- ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))
- 别名
- ELK1 (ELK1 产品)
- 别名
- Elk-1 antibody, Xelk antibody, Xelk-1 antibody, elk-1 antibody, elk1 antibody, ELK1, ETS transcription factor antibody, ELK1, member of ETS oncogene family antibody, ETS domain-containing protein Elk-1 antibody, ELK1, member of ETS oncogene family S homeolog antibody, ELK1 antibody, Elk1 antibody, Tsp_02690 antibody, elk1 antibody, elk1.S antibody
- 背景
- The transcription factor Elk1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk1. Elk1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 (Ser383) is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.Synonyms: ETS domain-containing protein Elk-1, Elk-1
- 基因ID
- 2002
- NCBI登录号
- NP_001107595
- UniProt
- P19419
- 途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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