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SOD1 抗体 (Internal Region)

Cited in 1 publication. This 山羊 多克隆 anti-SOD1 antibody specifically detects SOD1 in WB, ELISA 和 IF. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN190907
发货至: 中国
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Quick Overview for SOD1 抗体 (Internal Region) (ABIN190907)

抗原

See all SOD1 抗体
SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

适用

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人, 小鼠

宿主

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山羊

克隆类型

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多克隆

标记

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This SOD1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    SOD1

    序列

    SRKHGGPKDE ERH

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-SRKHGGPKDEERH, from the internal region of the protein sequence according to NP_000445.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 18 kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20 kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9 kDa according to Human NP_000445.1, Mous

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells and the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

    Additional validation: This antibody has been

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Frazziano, Al Ghouleh, Baust, Shiva, Champion, Pagano: "Nox-derived ROS are acutely activated in pressure overload pulmonary hypertension: indications for a seminal role for mitochondrial Nox4." in: American journal of physiology. Heart and circulatory physiology, Vol. 306, Issue 2, pp. H197-205, (2014) (PubMed).

  • 抗原

    SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

    别名

    SOD1

    背景

    SOD1, superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) , HGNC:11179, ALS, ALS1, IPOA, SOD , Cu /Zn superoxide dismutase, Cu/Zn superoxide dismutase, SOD, soluble, indophenoloxidase A, superoxide dismutase (aa 120-154), superoxide

    基因ID

    6647, 20655, 24786

    NCBI登录号

    NP_000445

    途径

    Sensory Perception of Sound, Transition Metal Ion Homeostasis
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