Nanog 抗体 (C-Term)
Quick Overview for Nanog 抗体 (C-Term) (ABIN190835)
抗原
See all Nanog (NANOG) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- NANOG (C Terminus)
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序列
- DLFLNYSMNM QPED
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-DLFLNYSMNMQPED, from the C Terminus of the protein sequence according to NP_079141.2.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2 μg/mL.
Western Blot: Approx 35 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6 kDa according to NP_079141.1). Recommended concentration: 0.05-0.1 μg/mL. An additional band of approx. 37 kDa is observed and is consistent with the obser
Peptide ELISA: antibody detection limit dilution 1:128000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Nanog (NANOG) (Nanog Homeobox (NANOG))
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别名
- NANOG
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背景
- NANOG, Nanog homeobox, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
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基因ID
- 79923
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NCBI登录号
- NP_079141
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途径
- Stem Cell Maintenance
抗原
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