Superoxide dismutase copper chaperone 抗体 (cleaved)
Quick Overview for Superoxide dismutase copper chaperone 抗体 (cleaved) (ABIN190735)
抗原
See all Superoxide dismutase copper chaperone (CCS) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- cleaved
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交叉反应 (详细)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
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预测反应
- dicots including: Glycine max, Solanum tuberosum
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 33.8 / 37 kDa (Arabidopsis thaliana)
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纯化方法
- serum
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免疫原
- The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.
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应用备注
- 1 : 1000 with alkaline phosphatase (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water.
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Superoxide dismutase copper chaperone (CCS) (Copper Chaperone For Superoxide Dismutase (CCS))
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别名
- CCS
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背景
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AGI Code: At1g12520
Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD. -
分子量
- expected: 33.8 kDa, apparent: 37 kDa (Arabidopsis thaliana)
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UniProt
- Q4ZJI5
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途径
- Transition Metal Ion Homeostasis
抗原
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