PRTN3 抗体
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- 抗原 See all PRTN3 抗体
- PRTN3 (Proteinase 3 (PRTN3))
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适用
- Nicotiana tabacum, Potato
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PRTN3 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- Antibody is recognizing closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa).
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 35, 34 / 32 and 34 kDa
- 纯化方法
- affinity purified
- 免疫原
- purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990, van Buuren et al., 1992): 1) Chitinase A (CHN A) P08252 encoded by gene chn48 derived from the N. tomentosiformis ancestor of tobacco. 2) Chitinase B (CHN B) P24091 encoded by gene chn50 derived from the N. sylvestris ancestor of tobacco.
- 亚型
- IgG
- Top Product
- Discover our top product PRTN3 Primary Antibody
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- 应用备注
- Recommended Dilution: 8 ug/mL with standard ECL (WB).
- 说明
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Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 100 µL of sterile water.
- 缓冲液
- PBS pH 7.4
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
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- 抗原
- PRTN3 (Proteinase 3 (PRTN3))
- 别名
- PR-3 (PRTN3 产品)
- 别名
- ela2 antibody, MGC132250 antibody, ACPA antibody, AGP7 antibody, C-ANCA antibody, CANCA antibody, MBN antibody, MBT antibody, NP-4 antibody, NP4 antibody, P29 antibody, PR-3 antibody, PR3 antibody, mPR3 antibody, proteinase 3 L homeolog antibody, proteinase 3 antibody, prtn3.L antibody, PRTN3 antibody, Prtn3 antibody
- 背景
- Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.
- 分子量
- expected: 35 kDa, 34 kDa, apparent: 32 and 34 kDa
- UniProt
- P08252, P24091
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