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PRTN3 抗体

The 兔 多克隆 anti-PRTN3 antibody is suitable to detect PRTN3 in samples from Agrostis stolonifera, Nicotiana tabacum, Picea abies, Potato, Red pepper, Tomato 和 Vitis vinifera. It has been validated for WB, IL 和 Co-IP.
产品编号 ABIN190718
发货至: 中国
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Quick Overview for PRTN3 抗体 (ABIN190718)

抗原

See all PRTN3 抗体
PRTN3 (Proteinase 3 (PRTN3))

适用

Agrostis stolonifera, Nicotiana tabacum, Picea abies, Potato, Red pepper, Tomato, Vitis vinifera

宿主

  • 61
  • 26
  • 1
  • 1
  • 1

克隆类型

  • 56
  • 34
多克隆

标记

  • 50
  • 10
  • 9
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
This PRTN3 antibody is un-conjugated

应用范围

  • 66
  • 35
  • 25
  • 16
  • 11
  • 7
  • 6
  • 6
  • 6
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunolocalization (IL), Co-Immunoprecipitation ( Co-IP)
  • 原理

    Anti-PR-3 / CHN | Class I chitinase

    特异性

    Antibody is recognizing closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa).

    交叉反应 (详细)

    Agostis stolonifera cv. 'Penncross', Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
    Not reactive in: Cicer arietinum

    预测反应

    Arabidopsis thaliana,Manihot esculenta,Zea mays

    纯化方法

    Protein G purified

    免疫原

    Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al.

    亚型

    IgG
  • 应用备注

    8 μg/mL (WB)

    说明

    Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 100 μL of sterile water

    缓冲液

    Total IgG. Protein G purified in PBS pH 7.4.

    注意事项

    Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

    储存条件

    -20 °C

    储存方法

    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • 抗原

    PRTN3 (Proteinase 3 (PRTN3))

    别名

    PR-3

    背景

    Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.

    Additional information: Antibody is recognizing closely related tobacco class I isoforms: endochitinase A CHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.

    分子量

    35, 34 | 32 and 34 kDa

    基因ID

    107759005

    UniProt

    P08252, P24091
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