PRTN3 抗体
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北京 101111
Quick Overview for PRTN3 抗体 (ABIN190718)
抗原
See all PRTN3 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-PR-3 / CHN | Class I chitinase
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特异性
- Antibody is recognizing closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa).
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交叉反应 (详细)
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Agostis stolonifera cv. 'Penncross', Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Not reactive in: Cicer arietinum -
预测反应
- Arabidopsis thaliana,Manihot esculenta,Zea mays
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纯化方法
- Protein G purified
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免疫原
- Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al.
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亚型
- IgG
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应用备注
- 8 μg/mL (WB)
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说明
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Important note: For blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water
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缓冲液
- Total IgG. Protein G purified in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- PRTN3 (Proteinase 3 (PRTN3))
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别名
- PR-3
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背景
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Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.
Additional information: Antibody is recognizing closely related tobacco class I isoforms: endochitinase A CHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.
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分子量
- 35, 34 | 32 and 34 kDa
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基因ID
- 107759005
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UniProt
- P08252, P24091
抗原
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