GCLC 抗体
(2 references)Quick Overview for GCLC 抗体 (ABIN190704)
抗原
See all GCLC 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antibody recognizes closely related tobacco class I isoforms: endochitinase ACHN-A (ca. 34 kDa) and endochitinase B CHN-B (ca. 32 kDa)
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交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
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预测反应
- dicots including:Pisum sativum, Riciuns communis, monocots including: Oryza sativa, Triticum aestivum
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产品特性
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Expected / apparent Molecular Weight of the Antigene: 50 kDa
This antibody can be used as a marker of vacuolar contents. -
纯化方法
- affinity purified
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免疫原
- KLH-conjugated synthetic peptide derived from Zea mays gamma-ECS Q8W4W3
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亚型
- IgG
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anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 528-637) antibody
GCLC 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3H1 unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibodyGCLC 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30) antibodyGCLC 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibodyGCLC 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 437-637) antibodyGCLC 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30), (N-Term) antibodyGCLC 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB31687 unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-252) antibodyGCLC 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 435-636) antibodyGCLC 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (Middle Region) antibodyGCLC 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 犬, 豚鼠, 马, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 6-34), (N-Term) antibodyGCLC 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1: 5000 with standard ECL (WB), 1: 5000 (IL)
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说明
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antibodies has been used in immunolocalization on Arabidopsis thaliana
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water.
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缓冲液
- PBS pH 7.4
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).
: "The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves." in: Planta, Vol. 182, Issue 1, pp. 43-51, (2013) (PubMed).
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: "The role of glutathione in mercury tolerance resembles its function under cadmium stress in Arabidopsis." in: Metallomics : integrated biometal science, Vol. 6, Issue 2, pp. 356-66, (2014) (PubMed).
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- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
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别名
- Gamma-ECS
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背景
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AGI Code: At4g23100
Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development. -
分子量
- 50 kDa
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UniProt
- Q8W4W3
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途径
- Cell RedoxHomeostasis
抗原
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