Suggested dilutions: Western blotting: 1.100-1.500 Immunohistochemistry 1.100-1.500
限制
仅限研究用
状态
Liquid
缓冲液
0.01 M PBS, pH 7.4 with 10 mg/ml BSA and 0.1 % Sodium azide
储存液
Sodium azide
注意事项
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
储存条件
-20 °C
储存方法
Store at -20 °C/1 year
有效期
12 months
抗原
Ki-67 (MKI67)
(Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
别名
Ki67
背景
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase).It is absent in resting (G0) cells.Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index).In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index.The correlation between low Ki67 index and histologically low grade tumours is strong.Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.Sueishi M.et al.J.Biol.Chem.275:28888-28892(2000) Takagi M.et al.J.Biol.Chem.276:25386-25391(2001) Beausoleil S.A.et al.Proc.Natl.Acad.Sci.U.S.A.101:12130-12135(2004) Olsen J.V.et al.Cell 127:635-648(2006) Synonyms: KIA, Ki-67, Ki67, MKI67