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PCK1 抗体 (C-Term)

Name: PCK1 抗体 (C-Term)
SKU: ABIN1882180
Availability: OutOfStock

This anti-PCK1 antibody is a 兔多克隆 antibody detecting PCK1 in WB, IHC (p) 和 IF. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 8+ publications.

Western blot analysis of PCK1 Antibody (C-term) (ABIN1882180 and ABIN2842170) in rat primary hepatocyte cell line lysates. (PCK1 抗体 (C-Term))

Immunohistochemical analysis of paraffin-embedded human kidney tissue using B performed on the Leica® BOND RXm. (PCK1 抗体 (C-Term))

IHC (p)

The anti-PCK1 Pab (ABIN1882180 and ABIN2842170) is used in Western blot to detect PCK1 in mouse liver (Lane 1) and mouse small intestine tissue lysates (Lane 2). (PCK1 抗体 (C-Term))

Confocal immunofluorescent analysis of PCK1 Antibody (C-term) (ABIN1882180 and ABIN2842170) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (PCK1 抗体 (C-Term))

All lanes : Anti-PCK1 Antibody (C-term) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Human kidney lysate Lane 3: NCI- whole cell lysate Lane 4: Mouse liver lysate Lane 5: Mouse kidney lysate Lane 6: Rat kidney lysate Lysates/proteins at 20 μg per lane. (PCK1 抗体 (C-Term))

5 images

(8 references)

产品编号 ABIN1882180

发货至: 中国

Quick Overview for PCK1 抗体 (C-Term) (ABIN1882180)

抗原

See all PCK1 抗体

PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This PCK1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

克隆位点

RB03646

抗原表位
15

10

8

4

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 592-622, C-Term

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1.

亚型

Ig Fraction
anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibody
PCK1 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 3E4 unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibody
PCK1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibody
PCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 312-563) antibody
PCK1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Middle Region) antibody
PCK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, Pig, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 330-379) antibody
PCK1 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, 猴, 小鸡, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (C-Term) antibody
PCK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 311-558) antibody
PCK1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibody
PCK1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (AA 1-622) antibody
PCK1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:2000. IHC-P-Leica: 1:250

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

有效期

6 months
Gummadi, Taylor, Curthoys: "Concurrent binding and modifications of AUF1 and HuR mediate the pH-responsive stabilization of phosphoenolpyruvate carboxykinase mRNA in kidney cells." in: American journal of physiology. Renal physiology, Vol. 303, Issue 11, pp. F1545-54, (2012) (PubMed).

Berger, Lindh, Wierup, Zmuda-Trzebiatowska, Lindqvist, Manganiello, Degerman: "Phosphodiesterase 3B is localized in caveolae and smooth ER in mouse hepatocytes and is important in the regulation of glucose and lipid metabolism." in: PLoS ONE, Vol. 4, Issue 3, pp. e4671, (2009) (PubMed).

Franckhauser, Muñoz, Elias, Ferre, Bosch: "Adipose overexpression of phosphoenolpyruvate carboxykinase leads to high susceptibility to diet-induced insulin resistance and obesity." in: Diabetes, Vol. 55, Issue 2, pp. 273-80, (2006) (PubMed).

Dunten, Belunis, Crowther, Hollfelder, Kammlott, Levin, Michel, Ramsey, Swain, Weber, Wertheimer: "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site." in: Journal of molecular biology, Vol. 316, Issue 2, pp. 257-64, (2002) (PubMed).

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, (2002) (PubMed).

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, (2002) (PubMed).

OBrien, Printz, Halmi, Tiesinga, Granner: "Structural and functional analysis of the human phosphoenolpyruvate carboxykinase gene promoter." in: Biochimica et biophysica acta, Vol. 1264, Issue 3, pp. 284-8, (1996) (PubMed).

Ting, Burgess, Chamberlain, Keith, Falls, Meisler: "Phosphoenolpyruvate carboxykinase (GTP): characterization of the human PCK1 gene and localization distal to MODY on chromosome 20." in: Genomics, Vol. 16, Issue 3, pp. 698-706, (1993) (PubMed).
抗原

PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

别名

PCK1

背景

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.

分子量

69195

NCBI登录号

NP_002582

UniProt

P35558

途径

Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis