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UCHL3 抗体 (C-Term)

Name: UCHL3 抗体 (C-Term)
SKU: ABIN1882147
Availability: OutOfStock

This anti-UCHL3 antibody is a 兔多克隆 antibody detecting UCHL3 in WB 和 IHC (p). Suitable for 人. This Primary Antibody has been cited in 4+ publications.

Western blot analysis of his tag antibody in UCHL3 gene transfected 293 lysates (35 μg/lane). (UCHL3 抗体 (C-Term))

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with UCHL3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (UCHL3 抗体 (C-Term))

IHC (p)

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with UCHL3 Antibody (C-term) (ABIN1882147 and ABIN2839167) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (UCHL3 抗体 (C-Term))

IHC (p)

Western blot analysis of UCHL3 (ABIN1882147 and ABIN2839167) in HepG2 cell line lysates (35 μg/lane). (UCHL3 抗体 (C-Term))

4 images

(4 references)

产品编号 ABIN1882147

发货至: 中国

Quick Overview for UCHL3 抗体 (C-Term) (ABIN1882147)

抗原

See all UCHL3 (Uchl3) 抗体

UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

适用

人

宿主

兔

克隆类型

多克隆

标记

This UCHL3 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB4187

Discover our top product Uchl3 Primary Antibody

抗原表位
15

14

7

7

3

2

2

2

2

1

1

1

1

1
AA 195-225, C-Term

预测反应

B, M, Pig, Rat

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This UCHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3.

亚型

Ig Fraction
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 131-230) antibody
Uchl3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4E9 unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 195-225) antibody
Uchl3 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 1-220) antibody
Uchl3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) antibody
Uchl3 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 1-220), (C-Term) antibody
Uchl3 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 131-230) antibody
Uchl3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (N-Term) antibody
Uchl3 适用: 人, 大鼠, 小鼠, Cow, 马, 犬, 兔, 豚鼠, 斑马鱼, Pig, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (C-Term) antibody
Uchl3 适用: 人, 大鼠, 小鼠, Cow, 马, 犬, 兔, Pig, 猴, Hamster IHC, IHC (p), IF, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (Internal Region) antibody
Uchl3 适用: 人, 大鼠, 小鼠, 犬, 小鸡 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 133-230) antibody
Uchl3 适用: 人, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. IHC-P: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

有效期

6 months
Henderson, Vij, Zeitlin: "Ubiquitin C-terminal hydrolase-L1 protects cystic fibrosis transmembrane conductance regulator from early stages of proteasomal degradation." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11314-25, (2010) (PubMed).

Sun, Ying, Li, Shang, He, Chen, Cheng, Zhou: "Role of UCH-L1/ubiquitin in acute testicular ischemia-reperfusion injury." in: Biochemical and biophysical research communications, Vol. 366, Issue 2, pp. 539-44, (2008) (PubMed).

Saito, Iida, Sekine, Kawauchi, Higuchi, Ogawa, Nakamura: "Catalog of 680 variations among eight cytochrome p450 ( CYP) genes, nine esterase genes, and two other genes in the Japanese population." in: Journal of human genetics, Vol. 48, Issue 5, pp. 249-70, (2003) (PubMed).

Wilkinson, Lee, Deshpande, Duerksen-Hughes, Boss, Pohl: "The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase." in: Science (New York, N.Y.), Vol. 246, Issue 4930, pp. 670-3, (1989) (PubMed).
抗原

UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

别名

UCHL3

背景

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.

分子量

26183

NCBI登录号

NP_001257881, NP_005993

UniProt

P15374

途径

Feeding Behaviour, Positive Regulation of fat Cell Differentiation