pan Keratin 抗体
Quick Overview for pan Keratin 抗体 (ABIN187832)
抗原
See all pan Keratin (panKRT) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes keratin Species Reactivity: Most mammals and birds.
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免疫原
- Keratin extracted from human skin. Antigen Location: Cytoplasm
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应用备注
- Immunohistochemistry - Frozen sections: Fixation for 3 min. with 1% formaldehyde is recommended. Formaldehyde-fixed, paraffin-embedded tissue sections: Proteolytic pretreatment with pronase or trypsine is recommended. Use at a 1:50 dilution in PBS or Tris. Recommended positive control: Squamous cell carcinoma, skin, cervix. The optimal dilution for a specific application should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 0.25 mL affinity chromatography purified liquid antiserum.
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储存条件
- 4 °C
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- pan Keratin (panKRT)
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别名
- Keratin
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背景
- This polyclonal antibody reacts with keratin of most mammals and birds. A positive reaction is obtained with all stratified squamous epithelia and in epidermal appendages such as hairfollicles and sebaceous glands. Also the epithelia of the urinary tract, stomach, small and large intestine, uterine endometrium, bile ducts, prostate, kidney collecting tubules, thymic reticulum cells, mesothelium and to a lesser extent hepatocytes, adenocarcinomas, transitional cell carcinomas and anaplastic carcinomas can be identified by this antiserum. Renal cell adenocarcinomas and small cell anaplastic lung tumors may remain negative when examined in paraffin sections. Hepatocytes and pancreatic acini show a positive staining. Nearly all cultured epithelial cells are positive, all non-epithelial cells are negative.
抗原
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