Flow cytometry: Use 2-5 µg/106 cells for intracellular staining. Immunoprecipitation: Use 2 µg/106 transfected cells. Western blot: Use at 1-3 µg/mL. Immunohistochemistry: (paraffin sections) Immunocytochemistry The optimal dilution for a specific application should be determined by the researcher.
限制
仅限研究用
状态
Liquid
缓冲液
100 µg of liquid Protein G purified monoclonal antibody at 0.5 mg/mL in PBS containing protein stabilizer and 0.05% sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. TLR3 cDNA codes for a protein, ~120 kDa. TLR3 has a restricted expression pattern being expressed in dendritic cells (DC). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes. The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.