GZMB 抗体
Quick Overview for GZMB 抗体 (ABIN187356)
抗原
See all GZMB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes human granzyme B. Species Reactivity: Human. Others not tested. Positive Control: Human CD8+ T cells.
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纯度
- 95 % as determined by SDS-PAGE.
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免疫原
- Recombinant human granzyme B (aa 7-227)
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亚型
- IgG2a
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anti-Granzyme B (GZMB) (AA 21-247) antibody
GZMB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) (AA 73-187) antibodyGZMB 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal GZMB-3014 unconjugated
anti-Granzyme B (GZMB) (AA 21-130) antibodyGZMB 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) (AA 1-247) antibodyGZMB 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) (AA 73-187) antibodyGZMB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal GZMB-3056 unconjugated
anti-Granzyme B (GZMB) antibodyGZMB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Granzyme B (GZMB) antibodyGZMB 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CLB-GB11 unconjugated
anti-Granzyme B (GZMB) (AA 21-247) antibodyGZMB 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 4E4 unconjugated
anti-Granzyme B (GZMB) (AA 21-247) antibodyGZMB 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2G12G7 unconjugated
anti-Granzyme B (GZMB) antibodyGZMB 适用: 人 ELISA, FACS, cELISA 宿主: 小鼠 Monoclonal GM-4C1 unconjugated
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应用备注
- Flow cytometry Immunocytochemistry Immunoprecipitation Immunohistochemistry: (frozen sections and paraffin sections) Western blot: Suggested dilution of 1 µg/mL The optimal dilution for a specific application should be determined by the researcher. Figure A: Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1 µg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. Figure B: Detection of granzyme B in a human CD8+ T cell clone. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20 °C for 5 min., then in acetone at -20 °C for 30 seconds. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hour at room temperature (RT) with 20 µg/mL of clone B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.
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限制
- 仅限研究用
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缓冲液
- 100 µg of liquid purified monoclonal antibody at 1 mg/mL in PBS with 0.02% sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- GZMB (Granzyme B (GZMB))
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背景
- Granzyme B is a 27 kDa serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and, assisted by perforin, enters the target cell. In the target, granzyme B processes and activates various pro-caspases, inducing apoptosis in the target cell.
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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