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Caspase 3 抗体 (AA 29-175) (FITC)

This anti-Caspase 3 antibody is a 兔 多克隆 antibody detecting Caspase 3 in WB, IHC, ICC 和 IF. Suitable for 大鼠. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN1858244
发货至: 中国

Quick Overview for Caspase 3 抗体 (AA 29-175) (FITC) (ABIN1858244)

抗原

See all Caspase 3 (CASP3) 抗体
Caspase 3 (CASP3)

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This Caspase 3 antibody is conjugated to FITC

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
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    AA 29-175

    原理

    FITC-Linked Polyclonal Antibody to Caspase 3 (CASP3)

    序列

    MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SG IYLDSSYKMD YPEMGLCIII NNKNFHKSTGMSARNGTDVD AANLRETFMA LKYEVRNKND LTREEIMELM DSVSKEDHSK RSSFVCVILS HGDEGVIFGT NGPVDLKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD

    特异性

    The antibody is a rabbit polyclonal antibody raised against CASP3. It has been selected for its ability to recognize CASP3 in immunohistochemical staining and western blotting.

    交叉反应

    人, 小鼠

    纯化方法

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    免疫原

    Recombinant CASP3 expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against CASP3 conjugated to fitc.

    亚型

    IgG
  • 应用备注

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    说明

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    500 μg/mL

    缓冲液

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    注意事项

    Avoid repeated freeze/thaw cycles

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • Chen, Cao, Asare, Lv, Zhu, Li, Wei, Gao, Zhang, Mao, Gao, Fan: "Amelioration of cardiac dysfunction and ventricular remodeling after myocardial infarction by danhong injection are critically contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms." in: Journal of ethnopharmacology, Vol. 194, pp. 559-570, (2016) (PubMed).

  • 抗原

    Caspase 3 (CASP3)

    别名

    Caspase 3

    背景

    CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1

    途径

    Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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