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GPX4 抗体 (C-Term)

This anti-GPX4 antibody is a 山羊 多克隆 antibody detecting GPX4 in WB, IHC, ELISA 和 IP. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN185669
发货至: 中国

Quick Overview for GPX4 抗体 (C-Term) (ABIN185669)

抗原

See all GPX4 抗体
GPX4 (Glutathione Peroxidase 4 (GPX4))

适用

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宿主

  • 62
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山羊

克隆类型

  • 48
  • 30
多克隆

标记

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This GPX4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

质量等级

Verified
  • 抗原表位

    • 11
    • 3
    • 3
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    • 1
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    • 1
    C-Term

    原理

    GPX4 (Isoform a and c)

    序列

    EEPLVIEKDL PHY

    特异性

    This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).

    交叉反应

    Cow, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-EEPLVIEKDLPHY, from the C Terminus of the protein sequence according to NP_002076.2, NP_001034937.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration: 0.3 μg/mL.

    Western Blot: Approx 19 kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1 kDa according to NP_002076.2). Recommended concentration: 0.1-0.3 μg/mL. This antibody has been successfully used in WB on

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunoprecipitation:This antibody has been successfully used in IP on Human: Schoenmakers E et al. (2010) PMID:21084748.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Papp, Lu, Bolderson, Boucher, Singh, Holmgren, Khanna: "SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress." in: Antioxidants & redox signaling, Vol. 12, Issue 7, pp. 797-808, (2010) (PubMed).

    Schoenmakers, Agostini, Mitchell, Schoenmakers, Papp, Rajanayagam, Padidela, Ceron-Gutierrez, Doffinger, Prevosto, Luan, Montano, Lu, Castanet, Clemons, Groeneveld, Castets, Karbaschi, Aitken, Dixon et al.: "Mutations in the selenocysteine insertion sequence-binding protein 2 gene lead to a multisystem selenoprotein deficiency disorder in humans. ..." in: The Journal of clinical investigation, Vol. 120, Issue 12, pp. 4220-35, (2010) (PubMed).

  • 抗原

    GPX4 (Glutathione Peroxidase 4 (GPX4))

    别名

    GPX4

    背景

    GPX4, glutathione peroxidase 4 (phospholipid hydroperoxidase) , HGNC:4556, PHGPx, snGPx, glutathione peroxidase 4, phospholipid hydroperoxidase, sperm nucleus glutathione peroxidase

    基因ID

    2879

    NCBI登录号

    NP_002076, NP_001034937
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