Hsc70 抗体 (Internal Region)
Quick Overview for Hsc70 抗体 (Internal Region) (ABIN185659)
抗原
See all Hsc70 (HSPA8) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- HSPA8 (Isoform 1)
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序列
- EKLQGKINDE DKQK
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特异性
- This antibody is expected to recognise isoform 1 (NP_006588.1) only.
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.
Western Blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9 kDa according to Human NP_006588.1 and Mouse NP_112442.2). This molecular weight is routinely observed by other sources. Recommended concentration:
Peptide ELISA: antibody detection limit dilution 1:4000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders." in: PLoS ONE, Vol. 4, Issue 9, pp. e7100, (2009) (PubMed).
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: "Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders." in: PLoS ONE, Vol. 4, Issue 9, pp. e7100, (2009) (PubMed).
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- Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
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别名
- HSPA8
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背景
- HSPA8 , heat shock 70 kDa protein 8 , HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71 , LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat sh
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基因ID
- 3312, 15481, 24468
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NCBI登录号
- NP_006588
抗原
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