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Hsc70 抗体 (Internal Region)

Cited in 1 publication. This 山羊 多克隆 anti-Hsc70 antibody specifically detects Hsc70 in WB, IHC, ELISA 和 IF. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN185659
发货至: 中国
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Quick Overview for Hsc70 抗体 (Internal Region) (ABIN185659)

抗原

See all Hsc70 (HSPA8) 抗体
Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

适用

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人, 小鼠

宿主

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山羊

克隆类型

  • 77
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多克隆

标记

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This Hsc70 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    HSPA8 (Isoform 1)

    序列

    EKLQGKINDE DKQK

    特异性

    This antibody is expected to recognise isoform 1 (NP_006588.1) only.

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9 kDa according to Human NP_006588.1 and Mouse NP_112442.2). This molecular weight is routinely observed by other sources. Recommended concentration:

    Peptide ELISA: antibody detection limit dilution 1:4000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Johnson, Kaplitt: "Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders." in: PLoS ONE, Vol. 4, Issue 9, pp. e7100, (2009) (PubMed).

  • 抗原

    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

    别名

    HSPA8

    背景

    HSPA8 , heat shock 70 kDa protein 8 , HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71 , LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat sh

    基因ID

    3312, 15481, 24468

    NCBI登录号

    NP_006588
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