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AIRE 抗体 (Internal Region)

Cited in 2+ publications. The 山羊 多克隆 anti-AIRE antibody (ABIN185643) specifically detects AIRE in WB, ELISA 和 IHC. The antibody is reactive with 人 和 Pig samples.
产品编号 ABIN185643
发货至: 中国
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中国
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Quick Overview for AIRE 抗体 (Internal Region) (ABIN185643)

抗原

See all AIRE 抗体
AIRE (Autoimmune Regulator (AIRE))

适用

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人, Pig

宿主

  • 48
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山羊

克隆类型

  • 53
多克隆

标记

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This AIRE antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    AIRE

    序列

    KDVDLSQPRK GRKP

    交叉反应

    Cow, 犬, 人, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-KDVDLSQPRKGRKP, from the internal region of the protein sequence according to NP_000374.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Thymus shows nuclear staining in select thymocytes. This antibody has been successfully used in IHC on Human: Ströbel P et al. (2014) PMID: 24236644. This antibody has been successfully used in IHC on Pig:

    Western Blot: Appr. 60 kDa in transfected HEK293 lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 0.5-1.0 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Suzuki, Suzuki, Nakai, Sembon, Fuchimoto, Onishi: "Transcriptional and histological analyses of the thymic developmental process in the fetal pig." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 63, Issue 2, pp. 215-25, (2014) (PubMed).

    Ströbel, Hartmann, Rosenwald, Kalla, Ott, Friedel, Schalke, Kasahara, Tomaru, Marx: "Corticomedullary differentiation and maturational arrest in thymomas." in: Histopathology, Vol. 64, Issue 4, pp. 557-66, (2014) (PubMed).

  • 抗原

    AIRE (Autoimmune Regulator (AIRE))

    别名

    AIRE

    背景

    AIRE, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) , AIRE1, APECED, APS1, APSI, PGA1 , autoimmune regulator (APECED protein), autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy),

    基因ID

    326

    NCBI登录号

    NP_000374

    途径

    Chromatin Binding
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