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GAPDH 抗体 (Internal Region)

Name: GAPDH 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN185543
Availability: OutOfStock

This anti-GAPDH antibody is a 山羊多克隆 antibody detecting GAPDH in WB, ELISA, IHC 和 IF. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 6+ publications.

ABIN185543 (0. (GAPDH 抗体 (Internal Region))

ABIN185543 (20 µg/mL) staining of PFA-perfused cryosection of Rat Liver. (GAPDH 抗体 (Internal Region))

ABIN185543 (2µg/ml) staining of paraffin embedded Human Liver. (GAPDH 抗体 (Internal Region))

IHC

ABIN185543 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0. (GAPDH 抗体 (Internal Region))

ABIN185543 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (GAPDH 抗体 (Internal Region))

11 images

(6 references)

产品编号 ABIN185543

发货至: 中国

Datasheet as PDF

Quick Overview for GAPDH 抗体 (Internal Region) (ABIN185543)

抗原

See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

适用

人, 小鼠, 大鼠

宿主

山羊

克隆类型

多克隆

标记

This GAPDH antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

质量等级

Verified

Discover our top product GAPDH Primary Antibody

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Internal Region

原理

GAPDH (Internal)

序列

GVNHEKYDNS LK

特异性

This antibody is expected to recognize both reported isoforms (NP_002037.2, NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels

交叉反应

犬, 人, 小鼠, 大鼠

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

免疫原

Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2.

亚型

IgG
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 斑马鱼 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal D1 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibody
GAPDH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2 μg/mL.
Western Blot: Approx 37 kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1 kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1
Peptide ELISA: antibody detection limit dilution 1:16000.

说明

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10μg/ml.

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Ramsden, Nommiste, R Lane, Carr, Powner, J K Smart, Chen, Muthiah, Webster, Moore, Cheetham, da Cruz, Coffey: "Rescue of the MERTK phagocytic defect in a human iPSC disease model using translational read-through inducing drugs." in: Scientific reports, Vol. 7, Issue 1, pp. 51, (2019) (PubMed).

Carr, Vugler, Yu, Semo, Coffey, Moss, Greenwood: "The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide." in: Molecular vision, Vol. 17, pp. 1701-15, (2011) (PubMed).

Carr, Vugler, Lawrence, Chen, Ahmado, Chen, Semo, Gias, da Cruz, Moore, Walsh, Coffey: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." in: Molecular vision, Vol. 15, pp. 283-95, (2009) (PubMed).

Chakir, Daya, Aiba, Tunin, Dimaano, Abraham, Jaques-Robinson, Jacques, Lai, Pacak, Zhu, Xiao, Tomaselli, Kass: "Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy." in: Circulation, Vol. 119, Issue 9, pp. 1231-40, (2009) (PubMed).

Schillace, Miller, Pisenti, Grotzke, Swarbrick, Lewinsohn, Carr: "A-kinase anchoring in dendritic cells is required for antigen presentation." in: PLoS ONE, Vol. 4, Issue 3, pp. e4807, (2009) (PubMed).

Vugler, Carr, Lawrence, Chen, Burrell, Wright, Lundh, Semo, Ahmado, Gias, da Cruz, Moore, Andrews, Walsh, Coffey: "Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation." in: Experimental neurology, Vol. 214, Issue 2, pp. 347-61, (2008) (PubMed).
抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

GAPDH

背景

GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase

基因ID

2597, 14433, 24383

NCBI登录号

NP_002037, NP_001243728