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14-3-3 sigma/SFN 抗体 (Internal Region)

This anti-14-3-3 sigma/SFN antibody is a 山羊 多克隆 antibody detecting 14-3-3 sigma/SFN in WB, IHC 和 ELISA. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN185539
发货至: 中国

Quick Overview for 14-3-3 sigma/SFN 抗体 (Internal Region) (ABIN185539)

抗原

See all 14-3-3 sigma/SFN (SFN) 抗体
14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

适用

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人, 小鼠, 大鼠

宿主

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山羊

克隆类型

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多克隆

标记

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This 14-3-3 sigma/SFN antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    14-3-3 sigma / Stratifin

    序列

    DLHTLSEDSY KDST

    交叉反应

    犬, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-DLHTLSEDSYKDST, from the internal region of the protein sequence according to NP_006133.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4 μg/mL.

    Western Blot: Approx 26-28 kDa band observed in Human, Mouse and Rat Skin lysates and in lysates of cell lines A431 and U2OS (calculated MW of 27.8 kDa according to Human NP_006133.1, Mouse NP_061224.2 and Rat XP_003750096.1). Recommended concentration: 0

    Peptide ELISA: antibody detection limit dilution 1:16000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

    别名

    SFN

    背景

    SFN, 14-3-3sigma, stratifin, HGNC:10773, YWHAS

    基因ID

    2810, 55948, 313017

    NCBI登录号

    NP_006133

    途径

    p53 Pathway, Myometrial Relaxation and Contraction
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