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PDE4D 抗体 (C-Term)

This 山羊 多克隆 antibody specifically detects PDE4D in WB, ELISA, IHC 和 EIA. It exhibits reactivity toward 人.It has been mentioned in 1 publication
产品编号 ABIN185530
发货至: 中国
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Quick Overview for PDE4D 抗体 (C-Term) (ABIN185530)

抗原

See all PDE4D 抗体
PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This PDE4D antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    PDE4D

    序列

    QPEACVIDDR SPDT

    特异性

    This antiobody is expected to recognize all reported isoforms.

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence QPEACVIDDRSPDT, from the C Terminus of the protein sequence according to NP_001184151.1, NP_001184150.1, NP_006194.2, NP_001098101.1, NP_001184152.1, NP_001159371.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 70+80+90 kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68 kDa and 95 kDa are observed. These bands are not observed in the untransfected COS1. Calculated

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol. 2010 Jan,47(4):678-84., PMID: 19939455.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Bazhin, Kahnert, Kimpfler, Schadendorf, Umansky: "Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells." in: Molecular immunology, Vol. 47, Issue 4, pp. 678-84, (2010) (PubMed).

  • 抗原

    PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

    别名

    PDE4D

    背景

    PDE4D, phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila), HGNC:8783, DPDE3, HSPDE4D, PDE4DN2, STRK1, cAMP-specific phosphodiesterase 4D, cAMP-specific phosphodiesterase PDE4D6, dunce-like phosphodiesterase E3, phosphodie

    基因ID

    5144

    NCBI登录号

    NP_001184151, NP_001184150, NP_006194, NP_001098101, NP_001184152, NP_001159371

    途径

    Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling
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