PDE4D 抗体 (C-Term)
(1 reference)Quick Overview for PDE4D 抗体 (C-Term) (ABIN185530)
抗原
See all PDE4D 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- PDE4D
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序列
- QPEACVIDDR SPDT
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特异性
- This antiobody is expected to recognize all reported isoforms.
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence QPEACVIDDRSPDT, from the C Terminus of the protein sequence according to NP_001184151.1, NP_001184150.1, NP_006194.2, NP_001098101.1, NP_001184152.1, NP_001159371.1.
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亚型
- IgG
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anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 164-213) antibody
PDE4D 适用: 人, 小鼠, 大鼠, 马, 小鸡, Pig, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (C-Term) antibodyPDE4D 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 466-709) antibodyPDE4D 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) antibodyPDE4D 适用: 人, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (C-Term) antibodyPDE4D 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 401-550) antibodyPDE4D 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 581-809) antibodyPDE4D 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) antibodyPDE4D 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 4D, cAMP-Specific (PDE4D) antibodyPDE4D 适用: 人 WB, ELISA 宿主: 兔 Monoclonal 4D4 unconjugated Recombinant Antibody
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应用备注
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Immunohistochemistry: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 5 μg/mL.
Western Blot: Approx 70+80+90 kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68 kDa and 95 kDa are observed. These bands are not observed in the untransfected COS1. Calculated
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol. 2010 Jan,47(4):678-84., PMID: 19939455.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells." in: Molecular immunology, Vol. 47, Issue 4, pp. 678-84, (2010) (PubMed).
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: "Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells." in: Molecular immunology, Vol. 47, Issue 4, pp. 678-84, (2010) (PubMed).
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- PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))
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别名
- PDE4D
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背景
- PDE4D, phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila), HGNC:8783, DPDE3, HSPDE4D, PDE4DN2, STRK1, cAMP-specific phosphodiesterase 4D, cAMP-specific phosphodiesterase PDE4D6, dunce-like phosphodiesterase E3, phosphodie
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基因ID
- 5144
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NCBI登录号
- NP_001184151, NP_001184150, NP_006194, NP_001098101, NP_001184152, NP_001159371
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途径
- Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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