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FZD8 抗体 (C-Term)

This 山羊 多克隆 anti-FZD8 antibody specifically detects FZD8 in WB, ELISA, IHC, IF 和 IEM. The antibody is reactive with 人 samples.
产品编号 ABIN185522
发货至: 中国
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Quick Overview for FZD8 抗体 (C-Term) (ABIN185522)

抗原

See all FZD8 抗体
FZD8 (Frizzled Family Receptor 8 (FZD8))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This FZD8 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoelectron Microscopy (IEM)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    FZD8 / frizzled 8

    序列

    SYPKQMPLSQ V

    交叉反应

    Cow, 犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-SYPKQMPLSQV, from the C Terminus of the protein sequence according to NP_114072.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 3 μg/mL.

    Western Blot: Approx 75 kDa band observed in Human Pancreas lysates (calculated MW of 73.3 kDa according to NP_114072.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse: Zhao B e

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: This antibody has been successfully used in IF on Mouse: Zhao B et al. (2019) PMID: 21321602.

    Additional validation: This antibody was successfully used in Electron microscopy (immunogold staining):

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    FZD8 (Frizzled Family Receptor 8 (FZD8))

    别名

    FZD8

    背景

    FZD8, frizzled homolog 8 (Drosophila) , HGNC:4046, FZ-8, hFZ8 , frizzled (Drosophila) homolog 8, frizzled 8

    基因ID

    8325, 14370

    NCBI登录号

    NP_114072

    途径

    WNT signaling
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