Clusterin 抗体 (C-Term)
Quick Overview for Clusterin 抗体 (C-Term) (ABIN185475)
抗原
See all Clusterin (CLU) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- Clusterin / APOJ
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序列
- EKALQEYRKK HREE
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特异性
- This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)
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交叉反应
- 犬, 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-EKALQEYRKKHREE, from the C Terminus of the protein sequence according to NP_001822.2, NP_976084.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 35-37 kDa band observed in Human Colon and Prostate lysates (calculated MW of 57.8 kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature (PMID: 9
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Clusterin (CLU)
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别名
- CLU
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背景
- CLU, APOJ, clusterin, complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J , HGNC:2095, CLI, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2 , apolipoprotein J, clusterin, complement-associ
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基因ID
- 1191
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NCBI登录号
- NP_001822, NP_976084
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途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
抗原
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