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Clusterin 抗体 (C-Term)

This 山羊 多克隆 anti-Clusterin antibody specifically detects Clusterin in WB, IHC, ELISA 和 IF. The antibody is reactive with 人 samples.
产品编号 ABIN185475
发货至: 中国
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Quick Overview for Clusterin 抗体 (C-Term) (ABIN185475)

抗原

See all Clusterin (CLU) 抗体
Clusterin (CLU)

适用

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宿主

  • 125
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山羊

克隆类型

  • 105
  • 78
  • 2
多克隆

标记

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This Clusterin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 10
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    C-Term

    原理

    Clusterin / APOJ

    序列

    EKALQEYRKK HREE

    特异性

    This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)

    交叉反应

    犬, 人

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-EKALQEYRKKHREE, from the C Terminus of the protein sequence according to NP_001822.2, NP_976084.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 35-37 kDa band observed in Human Colon and Prostate lysates (calculated MW of 57.8 kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature (PMID: 9

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Clusterin (CLU)

    别名

    CLU

    背景

    CLU, APOJ, clusterin, complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J , HGNC:2095, CLI, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2 , apolipoprotein J, clusterin, complement-associ

    基因ID

    1191

    NCBI登录号

    NP_001822, NP_976084

    途径

    Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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