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HRH2 抗体 (C-Term)

This anti-HRH2 antibody is a 山羊 多克隆 antibody detecting HRH2 in WB, ELISA, IF 和 FACS. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN185463
发货至: 中国

Quick Overview for HRH2 抗体 (C-Term) (ABIN185463)

抗原

See all HRH2 抗体
HRH2 (Histamine Receptor H2 (HRH2))

适用

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宿主

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山羊

克隆类型

  • 53
多克隆

标记

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This HRH2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    Histamine Receptor H2

    序列

    QEEKPLKLQV WSGTE

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-QEEKPLKLQVWSGTE, from the C Terminus of the protein sequence according to NP_071640.1, NP_001124527.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 50 kDa band observed in Human Tonsil lysates (calculated MW of 46.9 kDa according to Human NP_001354640.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Peng, Zhuang, He, Zhu, Wang: "Histamine excites neurons of the inferior vestibular nucleus in rats by activation of H1 and H2 receptors." in: Neuroscience letters, Vol. 541, pp. 87-92, (2013) (PubMed).

  • 抗原

    HRH2 (Histamine Receptor H2 (HRH2))

    别名

    HRH2

    背景

    HRH2, histamine receptor H2, HGNC:5183, H2R, gastric receptor 1, OTTHUMP00000161242

    基因ID

    3274

    NCBI登录号

    NP_001124527, NP_001354640
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