我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Nanog 抗体 (Internal Region)

Name: Nanog 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN185444
Availability: OutOfStock

This anti-Nanog antibody is a 山羊多克隆 antibody detecting Nanog in WB, ELISA, IF 和 IHC. Suitable for 人. This Primary Antibody has been cited in 10+ publications.

ABIN185444 (0. (Nanog 抗体 (Internal Region))

ABIN185444 (5 μg/mL) staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. (Nanog 抗体 (Internal Region))

2 images

(10 references)

产品编号 ABIN185444

发货至: 中国

Datasheet as PDF

Quick Overview for Nanog 抗体 (Internal Region) (ABIN185444)

抗原

See all Nanog (NANOG) 抗体

Nanog (NANOG) (Nanog Homeobox (NANOG))

适用

人

宿主

山羊

克隆类型

多克隆

标记

This Nanog antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

质量等级

Verified

Discover our top product NANOG Primary Antibody

抗原表位
16

16

10

9

9

7

7

6

5

5

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region

原理

NANOG

序列

QNQRMKSKRW QKNN

交叉反应

犬, 人, Pig

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

免疫原

Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.

亚型

IgG
anti-Nanog Homeobox (NANOG) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, ICC, IHC (p), IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 101-200) antibody
NANOG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-305) antibody
NANOG 适用: 人 WB, ELISA, IF, FACS, ICC 宿主: 小鼠 Monoclonal 8A1D11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 71-170) antibody
NANOG 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 15-49) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-100) antibody
NANOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 20-166) antibody
NANOG 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1E6C4 unconjugated

anti-Nanog Homeobox (NANOG) (AA 267-292) antibody
NANOG 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry: This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1,21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22,7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012,21(
Western Blot: Approx 38 kDa band observed in Human Ovary lysates (calculated MW of 34.6 kDa according to NP_079141.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:64000.

说明

IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green).
This antibody was successfully used in IF on Human: PMID: 30878013, 21681858, 22613719, 21877920. 21285354,19890879, 19483

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Sancho-Martinez, Nivet, Xia, Hishida, Aguirre, Ocampo, Ma, Morey, Krause, Zembrzycki, Ansorge, Vazquez-Ferrer, Dubova, Reddy, Lam, Hishida, Wu, Esteban, OLeary, Wahl, Verma, Laurent, Izpisua Belmonte: "Establishment of human iPSC-based models for the study and targeting of glioma initiating cells." in: Nature communications, Vol. 7, pp. 10743, (2016) (PubMed).

Tolar, McGrath, Xia, Riddle, Lees, Eide, Keene, Liu, Osborn, Lund, Blazar, Wagner: "Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa." in: The Journal of investigative dermatology, Vol. 134, Issue 5, pp. 1246-54, (2014) (PubMed).

Vassena, Montserrat, Carrasco Canal, Aran, de Oñate, Veiga, Izpisua Belmonte: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, (2012) (PubMed).

Montserrat, Ramírez-Bajo, Xia, Sancho-Martinez, Moya-Rull, Miquel-Serra, Yang, Nivet, Cortina, González, Izpisua Belmonte, Campistol: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, (2012) (PubMed).

Montserrat, de Oñate, Garreta, González, Adamo, Eguizábal, Häfner, Vassena, Izpisua Belmonte: "Generation of feeder-free pig induced pluripotent stem cells without Pou5f1." in: Cell transplantation, Vol. 21, Issue 5, pp. 815-25, (2012) (PubMed).

Montserrat, Garreta, González, Gutiérrez, Eguizábal, Ramos, Borrós, Izpisua Belmonte: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, (2011) (PubMed).

Montserrat, Bahima, Batlle, Häfner, Rodrigues, González, Izpisúa Belmonte: "Generation of pig iPS cells: a model for cell therapy." in: Journal of cardiovascular translational research, Vol. 4, Issue 2, pp. 121-30, (2011) (PubMed).

Eguizabal, Montserrat, Vassena, Barragan, Garreta, Garcia-Quevedo, Vidal, Giorgetti, Veiga, Izpisua Belmonte: "Complete meiosis from human induced pluripotent stem cells." in: Stem cells (Dayton, Ohio), Vol. 29, Issue 8, pp. 1186-95, (2011) (PubMed).

Rodríguez-Pizà, Richaud-Patin, Vassena, González, Barrero, Veiga, Raya, Izpisúa Belmonte: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, (2010) (PubMed).

Raya, Rodríguez-Pizà, Guenechea, Vassena, Navarro, Barrero, Consiglio, Castellà, Río, Sleep, González, Tiscornia, Garreta, Aasen, Veiga, Verma, Surrallés, Bueren, Izpisúa Belmonte: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, (2009) (PubMed).
抗原

Nanog (NANOG) (Nanog Homeobox (NANOG))

别名

NANOG

背景

NANOG, Nanog homeobox, HGNC:20857 , homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48

基因ID

79923, 71950, 414065

NCBI登录号

NP_079141

途径

Stem Cell Maintenance