HMGA1 抗体 (Internal Region)
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北京 101111
Quick Overview for HMGA1 抗体 (Internal Region) (ABIN185443)
抗原
See all HMGA1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- HMGA1
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序列
- LASKQEKDGT EK
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特异性
- This antibody is expected to recognise isoform a (also called HMG-I, NP_665906.1, NP_665908.1) and isoform b (also called HMG-Y, NP_002122.1, NP_665909.1, NP_665910.1, NP_665912.1).
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-LASKQEKDGTEK, from the internal region of the protein sequence according to NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1.
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亚型
- IgG
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anti-High Mobility Group AT-Hook 1 (HMGA1) antibody
HMGA1 适用: 人 WB, IHC, IP, IF, ChIP 宿主: 兔 Monoclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 51-107) antibodyHMGA1 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 64-93), (C-Term) antibodyHMGA1 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB17645 unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 64-93) antibodyHMGA1 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 9-21) antibodyVerified HMGA1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 1-96) antibodyHMGA1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 1-107) antibodyHMGA1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 71-101), (C-Term) antibodyHMGA1 适用: 人, 小鼠 WB, FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) (AA 1-100) antibodyHMGA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group AT-Hook 1 (HMGA1) antibodyHMGA1 适用: 人, 大鼠, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 4 μg/mL.
Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Testis and Ovary lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated si
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Additional validation: This antibody has been successfully used in qRT-PCR on Human: Cello et al. (2013) PMID: 23545254.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, (2013) (PubMed).
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: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, (2013) (PubMed).
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- HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))
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别名
- HMGA1
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背景
- HMGA1, high mobility group AT-hook 1 , HGNC:5010, HMG-R, HMGIY, MGC12816, MGC4242, MGC4854 , OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, HMGA1A,
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基因ID
- 3159
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NCBI登录号
- NP_665906, NP_665908, NP_002122, NP_665909, NP_665912, NP_665910
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途径
- Nuclear Hormone Receptor Binding, Autophagy
抗原
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