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HMGA1 抗体 (Internal Region)

This anti-HMGA1 antibody is a 山羊 多克隆 antibody detecting HMGA1 in IHC 和 ELISA. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN185443
发货至: 中国

Quick Overview for HMGA1 抗体 (Internal Region) (ABIN185443)

抗原

See all HMGA1 抗体
HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This HMGA1 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    HMGA1

    序列

    LASKQEKDGT EK

    特异性

    This antibody is expected to recognise isoform a (also called HMG-I, NP_665906.1, NP_665908.1) and isoform b (also called HMG-Y, NP_002122.1, NP_665909.1, NP_665910.1, NP_665912.1).

    交叉反应

    Cow, 犬, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-LASKQEKDGTEK, from the internal region of the protein sequence according to NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 4 μg/mL.

    Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Testis and Ovary lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated si

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Additional validation: This antibody has been successfully used in qRT-PCR on Human: Cello et al. (2013) PMID: 23545254.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Di Cello, Shin, Harbom, Brayton: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, (2013) (PubMed).

  • 抗原

    HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

    别名

    HMGA1

    背景

    HMGA1, high mobility group AT-hook 1 , HGNC:5010, HMG-R, HMGIY, MGC12816, MGC4242, MGC4854 , OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, HMGA1A,

    基因ID

    3159

    NCBI登录号

    NP_665906, NP_665908, NP_002122, NP_665909, NP_665912, NP_665910

    途径

    Nuclear Hormone Receptor Binding, Autophagy
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