OCT4 抗体 (C-Term)
Quick Overview for OCT4 抗体 (C-Term) (ABIN185438)
抗原
See all OCT4 (POU5F1) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- OCT4 / POU5F1
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序列
- C-VTTLGSPMHS N
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特异性
- This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-VTTLGSPMHSN, from the C Terminus of the protein sequence according to NP_002692.2, NP_976034.3.
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亚型
- IgG
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应用备注
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Western Blot: Approx 30 kDa band observed in Human Placenta, and approx 28 kDa in Mouse Heart lysates (calculated MW of 30.1 kDa according to Human NP_976034.3 and 25.0 kDa according to Mouse NP_001239381.1). Recommended concentration: 0.5-2 μg/mL. Primary in
Peptide ELISA: antibody detection limit dilution 1:2000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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别名
- POU5F1
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背景
- POU5F1, OTF4, POU domain, class 5, transcription factor 1, HGNC:9221, MGC22487, OCT3, OTF3, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3, POU class 5 homeobox 1, DADB-104B20.2, OCT4, POU domain, class 5, tran
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基因ID
- 5460, 18999, 294562
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NCBI登录号
- NP_002692, NP_976034
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途径
- Stem Cell Maintenance
抗原
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