NPY5R 抗体 (Internal Region)
Quick Overview for NPY5R 抗体 (Internal Region) (ABIN185409)
抗原
See all NPY5R 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Neuropeptide Y receptor Y5
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序列
- GLSNKENRLE EN
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交叉反应
- 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-GLSNKENRLEEN, from the internal region of the protein sequence according to NP_006165.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration, 4-6 μg/mL.
Western Blot: Approx 50 kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7 kDa according to NP_006165.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in W
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunoprecipitation: Customer feedback showed specific results when this product was used in IP. This antibody has been successfully used in IP: PMID: 30503694.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Cell Surface Protein Detection to Assess Receptor Internalization." in: Bio-protocol, Vol. 6, Issue 20, (2016) (PubMed).
: "Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line." in: Molecular cancer research : MCR, Vol. 8, Issue 4, pp. 604-14, (2010) (PubMed).
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: "Cell Surface Protein Detection to Assess Receptor Internalization." in: Bio-protocol, Vol. 6, Issue 20, (2016) (PubMed).
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- NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))
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别名
- NPY5R
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背景
- Neuropeptide Y receptor Y5 , HGNC:7958, NPY5R, NPYR5
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分子量
- 50.7kDa
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基因ID
- 4889, 18168, 25340
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NCBI登录号
- NP_006165
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途径
- Feeding Behaviour
抗原
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