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NPY5R 抗体 (Internal Region)

Cited in 2+ publications. This 山羊 多克隆 anti-NPY5R antibody specifically detects NPY5R in WB, IHC, ELISA 和 IP. The antibody is reactive with 人 和 Hamster samples.
产品编号 ABIN185409
发货至: 中国
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Quick Overview for NPY5R 抗体 (Internal Region) (ABIN185409)

抗原

See all NPY5R 抗体
NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

适用

  • 51
  • 9
  • 6
  • 3
  • 3
  • 2
人, Hamster

宿主

  • 50
  • 2
  • 1
山羊

克隆类型

  • 41
  • 12
多克隆

标记

  • 24
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This NPY5R antibody is un-conjugated

应用范围

  • 48
  • 18
  • 14
  • 13
  • 12
  • 11
  • 7
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

质量等级

Verified
  • 抗原表位

    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    Internal Region

    原理

    Neuropeptide Y receptor Y5

    序列

    GLSNKENRLE EN

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-GLSNKENRLEEN, from the internal region of the protein sequence according to NP_006165.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration, 4-6 μg/mL.

    Western Blot: Approx 50 kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7 kDa according to NP_006165.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in W

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunoprecipitation: Customer feedback showed specific results when this product was used in IP. This antibody has been successfully used in IP: PMID: 30503694.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Czarnecka, Kitlinska: "Cell Surface Protein Detection to Assess Receptor Internalization." in: Bio-protocol, Vol. 6, Issue 20, (2016) (PubMed).

    Sheriff, Ali, Yahya, Haider, Balasubramaniam, Amlal: "Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line." in: Molecular cancer research : MCR, Vol. 8, Issue 4, pp. 604-14, (2010) (PubMed).

  • 抗原

    NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

    别名

    NPY5R

    背景

    Neuropeptide Y receptor Y5 , HGNC:7958, NPY5R, NPYR5

    分子量

    50.7kDa

    基因ID

    4889, 18168, 25340

    NCBI登录号

    NP_006165

    途径

    Feeding Behaviour
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