HNF4A 抗体 (N-Term)
Quick Overview for HNF4A 抗体 (N-Term) (ABIN185378)
抗原
See all HNF4A 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- HNF4A
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序列
- RLSKTLVDMD MADY
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特异性
- This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1, NP_000448.3, NP_849181.1 resp.).
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交叉反应
- 犬, 人, Pig
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence RLSKTLVDMDMADY-C, from the N Terminus of the protein sequence according to NP_849180.1, NP_000448.3, NP_849181.1.
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亚型
- IgG
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anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) (AA 324-423) antibody
HNF4A 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 4E2 unconjugated
anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) (N-Term) antibodyHNF4A 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, 绵羊, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) (pSer313) antibodyHNF4A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) (AA 1-464) antibodyHNF4A 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) (AA 281-312) antibodyHNF4A 适用: 人, 大鼠 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) (AA 1-464) antibodyHNF4A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) (AA 200-300) antibodyHNF4A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) (C-Term) antibodyHNF4A 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) (Middle Region) antibodyHNF4A 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, 马, 兔, 豚鼠, 绵羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) (AA 281-312) antibodyHNF4A 适用: 人, 大鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB17745 unconjugated
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应用备注
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Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2 μg/mL.
Western Blot: Approx 55 kDa and 48 kDa bands observed in lysates of cell line HepG2 (calculated MW of 52.8 kDa according to Human NP_000448.3 , 46.6 kDa according to Human NP_849181.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:32000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
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别名
- HNF4A
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背景
- HNF4A, TCF, HNF4, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, hepatocyte nuclear factor 4, alpha, HNF4-alpha, transcription factor-14, FLJ39654, HNF4alpha, MODY, MODY1, HNF4alpha10/11/12, hepatic nuclear factor 4 alpha
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基因ID
- 3172
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NCBI登录号
- NP_849180, NP_000448, NP_849181
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途径
- AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process
抗原
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