PDE5A 抗体 (C-Term)
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Quick Overview for PDE5A 抗体 (C-Term) (ABIN185350)
抗原
See all PDE5A 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- PDE5A
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序列
- LINGESGQAK RN
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特异性
- This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
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交叉反应
- Cow, 犬, 人, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2, NP_246273.2, NP_236914.2, (NP_237223).
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亚型
- IgG
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anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 29-138) antibody
PDE5A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 9H5 unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (C-Term) antibodyPDE5A 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 101-200) antibodyPDE5A 适用: 人, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 1-150) antibodyPDE5A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (C-Term) antibodyPDE5A 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 20-63), (N-Term) antibodyPDE5A 适用: 人, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa according to isoform 2, NP_236914.2). Recommended concentrat
Peptide ELISA: antibody detection limit dilution 1:16000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
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别名
- PDE5A
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背景
- PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
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基因ID
- 8654
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NCBI登录号
- NP_001074, NP_246273, NP_236914
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途径
- Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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