PDE5A 抗体 (C-Term)
Quick Overview for PDE5A 抗体 (C-Term) (ABIN185350)
抗原
See all PDE5A 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
-
-
抗原表位
- C-Term
-
原理
- PDE5A
-
序列
- LINGESGQAK RN
-
特异性
- This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
-
交叉反应
- Cow, 犬, 人, 大鼠
-
纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
免疫原
- Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2, NP_246273.2, NP_236914.2, (NP_237223).
-
亚型
- IgG
-
-
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 29-138) antibody
PDE5A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 9H5 unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (C-Term) antibodyPDE5A 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 101-200) antibodyPDE5A 适用: 人, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (C-Term) antibodyPDE5A 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 1-150) antibodyPDE5A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 20-63), (N-Term) antibodyPDE5A 适用: 人, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western Blot: In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8 kDa according to isoform 2, NP_236914.2). Recommended concentrat
Peptide ELISA: antibody detection limit dilution 1:16000.
-
说明
-
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Minimize freezing and thawing.
-
储存条件
- -20 °C
-
储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
- PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
-
别名
- PDE5A
-
背景
- PDE5A, CN5A, PDE5, PDE5A1, CGB-PDE, phosphodiesterase 5A, cGMP-specific, cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
-
基因ID
- 8654
-
NCBI登录号
- NP_001074, NP_246273, NP_236914
-
途径
- Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
-
