TPI1 抗体 (C-Term)
Quick Overview for TPI1 抗体 (C-Term) (ABIN185349)
抗原
See all TPI1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- Triosephosphate isomerase
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序列
- LKPEFVDIIN AKQ
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特异性
- This antibody is expected to recognise reported isoforms 1, 2 and 3 (NP_000356.1, NP_001152759.1, NP_001244955.1.
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-LKPEFVDIINAKQ, from the C Terminus of the protein sequence according to NP_000356.1, NP_001152759.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Liver shows staining of the sinusoids. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Pancreas. Recommended concentration: 5 μg/mL.
Western Blot: Approx 26 kDa band observed in lysates of cell lines HEK293, HeLa, HepG2, Jurkat, Raw264.7. KNRK and MDCK (calculated MW of 26.7 kDa according to Human NP_000356.1). Recommended concentration: 0.001-0.01 μg/mL. Primary incubation 1 hour at roo
Peptide ELISA: antibody detection limit dilution 1:16000.
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说明
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Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53." in: Proteome science, Vol. 6, pp. 18, (2008) (PubMed).
: "Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis--a proteomic study." in: Proteome science, Vol. 5, pp. 5, (2007) (PubMed).
: "
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Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53." in: Proteome science, Vol. 6, pp. 18, (2008) (PubMed).
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- TPI1 (Triosephosphate Isomerase 1 (TPI1))
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别名
- TPI1
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背景
- TPI1, triosephosphate isomerase 1, TPI, MGC88108
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基因ID
- 7167, 21991, 24849
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NCBI登录号
- NP_000356, NP_001152759
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途径
- Cell RedoxHomeostasis
抗原
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