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TPI1 抗体 (C-Term)

Cited in 2+ publications. The 山羊 多克隆 anti-TPI1 antibody (ABIN185349) specifically detects TPI1 in WB, ELISA, IHC 和 FACS. The antibody is reactive with 人, 小鼠, 大鼠 和 犬 samples.
产品编号 ABIN185349
发货至: 中国
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Quick Overview for TPI1 抗体 (C-Term) (ABIN185349)

抗原

See all TPI1 抗体
TPI1 (Triosephosphate Isomerase 1 (TPI1))

适用

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人, 小鼠, 大鼠, 犬

宿主

  • 62
  • 10
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  • 1
山羊

克隆类型

  • 59
  • 19
多克隆

标记

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This TPI1 antibody is un-conjugated

应用范围

  • 74
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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 15
    • 8
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
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    • 1
    • 1
    C-Term

    原理

    Triosephosphate isomerase

    序列

    LKPEFVDIIN AKQ

    特异性

    This antibody is expected to recognise reported isoforms 1, 2 and 3 (NP_000356.1, NP_001152759.1, NP_001244955.1.

    交叉反应

    Cow, 犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-LKPEFVDIINAKQ, from the C Terminus of the protein sequence according to NP_000356.1, NP_001152759.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Liver shows staining of the sinusoids. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Pancreas. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 26 kDa band observed in lysates of cell lines HEK293, HeLa, HepG2, Jurkat, Raw264.7. KNRK and MDCK (calculated MW of 26.7 kDa according to Human NP_000356.1). Recommended concentration: 0.001-0.01 μg/mL. Primary incubation 1 hour at roo

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Honoré, Buus, Claësson: "Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53." in: Proteome science, Vol. 6, pp. 18, (2008) (PubMed).

    Vorum, Østergaard, Rice, Honoré, Bek: "Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis--a proteomic study." in: Proteome science, Vol. 5, pp. 5, (2007) (PubMed).

  • 抗原

    TPI1 (Triosephosphate Isomerase 1 (TPI1))

    别名

    TPI1

    背景

    TPI1, triosephosphate isomerase 1, TPI, MGC88108

    基因ID

    7167, 21991, 24849

    NCBI登录号

    NP_000356, NP_001152759

    途径

    Cell RedoxHomeostasis
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