PD-L1 抗体 (C-Term)
(1 reference)Quick Overview for PD-L1 抗体 (C-Term) (ABIN185339)
抗原
See all PD-L1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- CD274 / PD-L1
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序列
- CKKQSDTHLE ET
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特异性
- This antibody is expected to recognize reported isoforms a and b (NP_054862.1, NP_001254635.1) only.
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence CKKQSDTHLEET, from the C Terminus of the protein sequence according to NP_054862.1.
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亚型
- IgG
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anti-CD274 (PD-L1) (AA 19-238) antibody
PD-L1 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 9B5F4 unconjugated
anti-CD274 (PD-L1) (AA 39-191) antibodyPD-L1 适用: 人, 小鼠 WB, FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal PDL1-2746 unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 6D9A2 unconjugated
anti-CD274 (PD-L1) (AA 48-290) antibodyPD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 34-241) antibodyPD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 19-238) antibodyPD-L1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (C-Term) antibodyKO Validated PD-L1 适用: 人 WB, FACS, IF, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 7D2A10 unconjugated
anti-CD274 (PD-L1) (AA 201-290) antibodyPD-L1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 24-234) antibodyPD-L1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx 50 kDa band observed in Human Heart lysates and in lysates of cell line U2OS (calculated MW of 33.3 kDa according to 1NP_054862.1).This band was successfully blocked by incubation with the immunizing peptide and is routinely observed
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in th membrane and cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Upregulation of B7-H1 expression is associated with macrophage infiltration in hepatocellular carcinomas." in: Cancer immunology, immunotherapy : CII, Vol. 61, Issue 1, pp. 101-8, (2012) (PubMed).
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: "Upregulation of B7-H1 expression is associated with macrophage infiltration in hepatocellular carcinomas." in: Cancer immunology, immunotherapy : CII, Vol. 61, Issue 1, pp. 101-8, (2012) (PubMed).
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- PD-L1 (CD274 (PD-L1))
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别名
- CD274
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背景
- CD274, CD274 antigen, PD-L1, PDCD1LG1, B7-H, B7H1, PDL1, PDCD1L1, programmed cell death 1 ligand 1, PDL1, HGNC:17635, CD274 Molecule, MGC142294, MGC142296
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基因ID
- 29126
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NCBI登录号
- NP_054862, NP_001254635
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途径
- Cancer Immune Checkpoints
抗原
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