PPARD 抗体 (C-Term)
Quick Overview for PPARD 抗体 (C-Term) (ABIN185282)
抗原
See all PPARD 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- PPAR delta (Isoform 1)
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序列
- CHPLLQEIYK DMY
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特异性
- This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.
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交叉反应
- Cow, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence CHPLLQEIYKDMY, from the C Terminus of the protein sequence according to NP_006229.1.
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亚型
- IgG
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应用备注
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Western Blot: Approx 55 kDa band observed in T cell line MOLT4 lysates (calculated MW of 49.9 kDa according to NP_006229.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:32000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Iron restriction improves type 2 diabetes mellitus in Otsuka Long-Evans Tokushima fatty rats." in: American journal of physiology. Endocrinology and metabolism, Vol. 298, Issue 6, pp. E1140-9, (2010) (PubMed).
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Iron restriction improves type 2 diabetes mellitus in Otsuka Long-Evans Tokushima fatty rats." in: American journal of physiology. Endocrinology and metabolism, Vol. 298, Issue 6, pp. E1140-9, (2010) (PubMed).
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- PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))
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别名
- PPARD
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背景
- PPARD, FAAR, NUC1, NUCI, NR1C2, NUCII, PPARB, MGC3931, PPAR-beta, peroxisome proliferative activated receptor, delta, nuclear hormone receptor 1, peroxisome proliferative activated receptor delta, OTTHUMP00000016256, OTTHUMP00000016257
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分子量
- 49.9kDa
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基因ID
- 5467
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NCBI登录号
- NP_006229
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途径
- Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Monocarboxylic Acid Catabolic Process, Smooth Muscle Cell Migration, Positive Regulation of fat Cell Differentiation
抗原
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