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FTCD 抗体 (N-Term)

There is 1 publication for this product available. The 山羊 多克隆 anti-FTCD antibody is suitable to detect FTCD in samples from 人. It has been validated for WB, ELISA, FACS 和 IF.
产品编号 ABIN185267
发货至: 中国
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Quick Overview for FTCD 抗体 (N-Term) (ABIN185267)

抗原

See all FTCD 抗体
FTCD (Formiminotransferase Cyclodeaminase (FTCD))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This FTCD antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    N-Term

    原理

    58K Golgi protein(N-Term)/FTCD

    序列

    SQLVECVPNF SEGKNQ

    特异性

    Reported variants represent identical protein: NP_996848.1, NP_006648.1

    交叉反应

    人, 小鼠, Pig

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence SQLVECVPNFSEGKNQ, from the N Terminus of the protein sequence according to NP_006648.1, NP_996848.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 58-60 kDa band observed in Human Liver lysates and in lysates of cell line HepG2 (calculated MW of 58.9 kDa according to NP_006648.1). Recommended concentration: 0.05-0.1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Hagiwara, Tajika, Matsuzaki, Suzuki, Aoki, Takata: "Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome." in: Histochemistry and cell biology, Vol. 126, Issue 2, pp. 251-9, (2006) (PubMed).

  • 抗原

    FTCD (Formiminotransferase Cyclodeaminase (FTCD))

    别名

    FTCD

    背景

    FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferase cyclodeaminase

    基因ID

    10841, 14317

    NCBI登录号

    NP_006648
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